患暴发性败血症中华鳖体内细菌的分离与鉴定  

Isolation and identification of bacteria from soft-shelled turtle (Trionyx sinensis) associated with fulminant septicaemia

作　　者：胡广洲[1] 李登峰[1] 苏秀榕[1] 李太武[1] 贺静静[1] 王孟前[1] 李慧[1] 李晔[1] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211

出　　处：《中国水产科学》2010年第4期859-868,共10页Journal of Fishery Sciences of China

基　　金：国家自然科学基金项目(30840061);宁波大学学科项目(XK0715049);宁波大学人才项目(XR0713017)

摘　　要：从患暴发性出血性败血症中华鳖(Trionyx sinensis)的肠、心、肝、胃等组织和腹腔液中分离培养得到8株细菌。通过光镜与电镜观察和16S rDNA序列测定、比对,对所分离细菌进行鉴定。结果表明,它们分别为格氏乳球菌(Lactococcus garvieae)、假单胞菌(Pseudomonas sp.)、海氏肠球菌(Enterococcus hirae)、屎肠球菌(Enterococcus faecium)、人苍白杆菌(Ochrobactrum anthropi)、惰性嗜血杆菌(Haemophilus segnis)、弗氏柠檬酸杆菌(Citrobacter freundii)和迟钝爱德华氏菌(Edwardsiella tarda)。用含抗生素的20种药敏纸片对格氏乳球菌、弗氏柠檬酸杆菌和迟钝爱德华氏菌进行药敏试验发现,格氏乳球菌对青霉素等4种药物敏感,对头孢唑啉等6种药物中度敏感,对阿米卡星等10种药物有耐药性。弗氏柠檬酸杆菌对头孢唑啉等13种药物敏感,对青霉素等3种药物中度敏感,对万古霉素等4种药物有耐药性。迟钝爱德华氏菌对新霉素等3种药物敏感,对阿米卡星等5种药物中度敏感,对青霉素等12种药物有耐药性。本研究旨在为中华鳖败血症的防治技术提供科学支撑。Eight bacteria were isolated from sick soft-shelled turtles（Trionyx sinensis）associated with fulminant septicaemia. Morphology of the bacteria was characterized by LM,SEM and TEM. 16S rDNA of the bacteria were amplified and sequenced. Results showed that they were Lactococcus garvieae,Pseudomonas sp.,Enterococcus hirae,Enterococcus faecium,Ochrobactrum anthropii,Haemophilus segnis,Citrobacter freundii and Edwardsiella tarda. Antibiotic sensitivity patterns of L. garvieae,C. freundii and E. tarda were evaluated against 20 antibiotics. L. garvieae was sensitive to 4 antibiotics including Penicillin,and was moderately sensitive to 6 antibiotics including Cefazolin,but was resistant to 10 antibiotics including Amilkacin. C. freundii was sensitive to 13 antibiotics including Cefazolin,and was moderately sensitive to 3 antibiotics including Penicillin,but was resistant to 4 antibiotics including Vancomycin. E. tarda was sensitive to 3 antibiotics including Neomycin,and was moderately sensitive to 5 antibiotics including Amilkacin,but was resistant to 12 antibiotics including Penicillin.

关 键 词：中华鳖 败血症 细菌 16SrDNA 药敏试验 

分 类 号：S94[农业科学—水产养殖]