复方参芪合剂治疗实验性自身免疫性重症肌无力大鼠模型的研究  

Behavioral and immunological study on experimental autoimmune myasthenia gravis treated with Shenqi Chinese herb drug mixture

作　　者：邵锦根[1] 叶好好[1] 李佳[1] 张旭[1] 

机构地区：[1]温州医学院附属第一医院神经内科,325000

出　　处：《中国神经免疫学和神经病学杂志》2010年第4期242-246,共5页Chinese Journal of Neuroimmunology and Neurology

基　　金：浙江省巾医药科研基金资助项目(2005C092)

摘　　要：目的研究复方参芪合剂治疗实验性自身免疫性重症肌无力(EAMG)的有效性及其可能机制。方法将经纯化的电鳐乙酰胆碱受体(AChR)免疫的Lewis大鼠30只随机分为对照组、复方参芪组和地塞米松组(DXM)3组,每组各10只。治疗前后分别对3组鼠进行行为学评分,以放射免疫法检测血清AChR抗体(IgG)水平,ELISA法检测抗体亚型IgG1、IgG2a水平,以流式细胞技术检测各种细胞因子分泌型细胞的比例。结果 (1)行为学评分:复方参芪组与DXM组分别为(0.42±0.31)分和(0.26±0.15)分,两组间无统计学差异(P>0.05),但均低于对照组[(0.83±0.43)分](P<0.05);(2)AChR抗体水平:复方参芪组[(16.26±2.31)nmol/L]低于对照组[(24.15±2.45)nmol/L],但高于DXM组[(12.41±2.92)nmol/L](均P<0.01)。(3)细胞因子分泌型细胞比例:IFN-γ及IL-4分泌型细胞比例复方参芪组[分别为(32.48±5.32)%、(21.58±7.17)%]和地塞米松组[分别为(24.74±3.19)%、(20.40±4.13)%]均低于对照组[分别为(39.20±4.17)%、(29.12±4.87)%](分别P<0.01、P<0.05),复方参芪组IFN-γ分泌型细胞比例高于地塞米松组(P<0.05),但两组间IL-4分泌型细胞比例差异无统计学意义(P>0.05);IL-10分泌型细胞比例,复方参芪组[(30.60±7.04)%]分别高于对照组[(23.90±4.72)%]和地塞米松组[(18.47±5.29)%](均P<0.05),地塞米松组与对照组间无统计学差异(P>0.05)。结论复方参芪合剂对EAMG有一定疗效,其机制可能与调节Th1/Th2细胞比例、促进免疫平衡有关。Objective To explore the therapeutic effect and possible mechanism of Shenqi mixture on experimental autoimmune myasthenia gravis （EAMG） treatment. Methods Thirty Lewis rats were induced by purified acetylcholine reeeptors （AChR） extracted from the electric organs of electric rays. Then the rats were divided into three groups randomly. The control group were treated with phosphate buffered saline （PBS）, the DXM group were treated with dexamethasone as routinely treated control, and the Shenqi group were treated with Shenqi mixture. All rats were weighted and examined daily for scores of behaviors before and after the treatment. The levels of sera AChR antibody were determined with radioimmunoassay （RIA）, and the levels of antibodies IgG1 and IgG2a were measured by ELISA, the percentages of lymphocytes to secret interferon-γ （IFN 7）, interleukin-4 （IL-4） and interleukin-10 （IL-10） were analyzed by flow cytometry. Results （1） Compared with the control group （0.83±0.43）, the scores of behaviors of the Shenqi group （0.42±0.31） and the DXM group （0.26±0. 15） were markedly lower （P〈0.05）. There was no significant difference between the Shenqi group and the DXM group （P〉0.05）. （2） The levels of sera AChR antibody of the Shenqi group [ （16.26±2.31） nmol/L] were lower than that of the control group [ （24.15±2.45） nmol/L] （P〈0.01）, but higher than that of the DXM group [ （12.41±2.92） nmol/L] （P〈0. 01）. （3） The percentages of tymphocytes to secret IFN-γ and IL-4 in the Shenqi group [ （32.48 ± 5.32）%, （21.58 ± 7.17）%] and the DXM group （24.74 ±3.19）%, （20.40±4.13）% ~ were both lower than that in the control group [ （39.20±4. 17）%,（29.12±4.87） %] （P〈0.01, P〈0.05 respectively ）, and the percentage of lymphocytes to secret IFN-γ in the Shenqi group was higher than that of the DXM group （P〈0.01）, but there was no significant difference of the percentage of lymphocytes to secret IL-4 betwee

关 键 词：实验性自身免疫性重症肌无力 复方参芪合剂 治疗 免疫调节 

分 类 号：R741.05[医药卫生—神经病学与精神病学]