核因子-κB抑制剂对偏头痛患者血清培养正常人单核细胞株表达的血清促炎细胞因子的影响  

Effect of NF-κB inhibitor on the levels of pro-inflammatory TNF-α and IL-17 in the normal monocyte cell line cultured by the serum of patients with migraine

作　　者：梅海云[1] 杨晓苏[1] 

机构地区：[1]中南大学湘雅医院神经内科,湖南省长沙市410008

出　　处：《国际神经病学神经外科学杂志》2010年第3期215-217,共3页Journal of International Neurology and Neurosurgery

摘　　要：目的观察偏头痛患者血清培养细胞后,细胞上清液中所产生的肿瘤坏死因子α(TNF-α)、白介素-17(IL-17)的水平,以及应用NF-κB抑制剂BAY11-7082后二者含量的变化,探讨TNF-α、IL-17、NF-κB在偏头痛的发生发展中的关系。方法选取偏头痛发作期患者及正常对照者各40例,采取单侧颈静脉血血清。采用人单核细胞株THP-1进行培养,将THP-1随机分成偏头痛血清组、偏头痛血清+NF-κB抑制剂组及正常血清对照组。各组血清培养THP-124h后,分别收集细胞上清液,采用双抗体酶联免疫吸附法检测上清液中TNF-α、IL-17的水平。结果偏头痛组血清培养上清液中TNF-α、IL-17水平高于正常血清对照组,差异有统计学意义(P<0.05);偏头痛血清+NF-κB抑制剂组上清液中TNF-α含量低于偏头痛血清组,差异有统计学意义(P<0.05),和正常血清组相比差异无统计学意义;该组上清液中IL-17含量低于偏头痛血清组,但差异无统计学意义(P>0.05),但仍高于正常血清组,差异具有统计学意义(P<0.05)。相关分析显示3组TNF-α与IL-17水平均呈正相关。结论偏头痛患者发作期可能存在NF-κB导致的促炎性细胞因子TNF-α、IL-17的分泌增加。Objective To study the levels of pro-inflammatory tumor necrosis factor alpha （TNF-α） and interleukin 17 （IL-17） in the supernatant which was from monocyte cell line cultured by the serum of patients with migraine in vitro,and study the effect of nuclear factor kappa B （NF-κB） inhibitor on the two pro-inflammatory cytokines.Methods Serums were collected from 40 migraine patients at the headache period and 40 normal persons （control group）. Monocyte cell line THP-1 was cultured in 10% fetal calf serum plus 1640 medium which contained 100 U/ml penicillin and 100 μg/ml streptomycin. The THP-1 cells were divided into 3 groups： normal control group,patient’s serum group and patient’s serum ＋ NF-κB inhibitor BAY11-7082 group. After the cells had been cultured for 24 hrs the levels of TNF-α and IL-17 were measured using ELISA.Results After the cells been cultured for 24 hrs the serum levels of TNF-α and IL-17 in the culture supernatant of the THP-1 cells from the migraineur group were higher than those from the control group （P〈0.05）. The levels of TNF-α from the patient’s serum ＋ BAY11-7082 group were lower than the patient’s serum group （P〈0.05） and were similar to the normal control group. The levels of IL-17 in the patient’s serum ＋ BAY11-7082 group were lower than those in the patient’s serum group,but there were no significant differences. Compared with the normal control group,the levels of IL-17 in the patient’s serum ＋ BAY11-7082 group increased significantly （P〈0.05）. The correlation analysis revealed that TNF-α levels were significantly positively correlated with IL-17 levels in all of the three groups.Conclusions At the stage of migraine attacks NF-κB might promote the secretion of pro-inflammatory factors TNF-α and IL-17 in patients with migraine.

关 键 词：偏头痛 核因子-κB 单核细胞株 肿瘤坏死因子α 白介素-17 NF-ΚB抑制剂 

分 类 号：R747.2[医药卫生—神经病学与精神病学]