卵巢癌组织中MDR-1、Wnt-1、FZD-1基因的表达变化及其与化疗耐药的关系  

Change and relationship of expression of MDR-1,Wnt-1 and FZD-1 genes in ovarian cancer tissue with drug-resistance

作　　者：刘博文[1] 李红霞[1] 

机构地区：[1]北京世纪坛医院,北京100038

出　　处：《山东医药》2010年第26期1-3,共3页Shandong Medical Journal

基　　金：国家"十一五"863计划基金资助项目(2006AA02A245)

摘　　要：目的观察化疗及非化疗卵巢癌组织中多药耐药基因-1(MDR-1)及Wnt-1、FZD-1基因的表达变化,探讨其与卵巢癌化疗耐药的关系。方法卵巢癌患者60例,包括紫杉醇化疗组(Ⅰ组)和未化疗组(Ⅱ组)各30例,Ⅰ组分为先期化疗有效组(ⅠA组)22例和化疗后复发组(ⅠB组)8例。采用实时荧光定量RT-PCR技术检测各组卵巢癌组织中的MDR-1、Wnt-1、FZD-1基因。结果Ⅰ组MDR-1基因阳性表达率为70%(21/30),Ⅱ组为86%(26/30),两组相比,P>0.05;ⅠB组MDR-1阳性6例(6/8),较ⅠA组的15例(15/22)略高,但两组相比,P>0.05。Wnt-1基因在MDR-1表达阳性的卵巢癌组织中的Ct值为4.742 9±0.756 9,与MDR-1阴性者的3.298 1±1.489 7相比,P<0.01。FZD-1基因在MDR-1表达阳性的卵巢癌组织中的Ct值为3.677 3±1.220 8,与MDR-1阴性者的4.898 0±0.633 9相比,P<0.01。在Ⅰ组中,MDR-1和Wnt-1、FZD-1基因的表达呈正相关(r分别为0.525、0.678,P均<0.05);但在Ⅱ组中,MDR-1和Wnt-1、FZD-1基因的表达无相关性(r分别为-0.091、0.031,P均>0.05)。结论卵巢癌组织中MDR-1基因有较高的阳性表达率,与卵巢癌的化疗耐药有关,MDR-1基因可能通过抑制Wnt-1、FZD-1基因的表达而发挥作用。Objective To investigate the expression alteration of MDR-1,Wnt-1 and FZD-1 in ovarian cancer tissues and the relationship with drug-resistance.Methods 60 cases of ovarian cancer were divided into two groups： taxol chemotherapy group（groupⅠ,30 cases）and not received chemotherapy group（groupⅡ,30 cases）.GroupⅠwas further divided into two subgroups： advance chemotherapy effective group（groupⅠA） and recrudescent group（groupⅠB）.MDR-1,Wnt-1 and FZD-1 genes in each group were detected by realtime RT-PCR.Results The positive rate of expression of MDR-1 gene in groupⅠand groupⅡwere 70%（21/30）and 86%（26/30） respectively,P〉0.05.MDR-1 gene was positive in 6 cases of groupⅠB（6/8） and 15 cases of groupⅠA（15/22）,P〈0.05.The Ct value of Wnt-1 gene in cases with both MDR-1 positive and non-positive were 4.742 9±0.756 9 and 3.298 1±1.489 7,P〈0.01.The Ct value of FZD-1gene in cases with MDR-1 positive and non-positive were 3.677 3±1.220 8,4.898 0±0.633 9,P〈0.01.The expression of MDR-1 had a positive correlation with Wnt-1 and FZD-1 in groupⅠ（r were 0.525 and 0.678,P〈0.05 respectively）.However,the expression of MDR-1 had no correlation with the other two genes in groupⅡ（r were-0.091 and 0.031,P〈0.05 respectively）.Conclusions The positive rate of expression of MDR-1 in ovarian cancer tissues are high,which is related to drug-resistance.The mechanism may be that MDR-1 can inhibit the expression of Wnt-1,FZD-1 gene.

关 键 词：卵巢肿瘤 化疗耐药 MDR-1基因 Wnt-1基因 FZD-1基因 

分 类 号：R737.31[医药卫生—肿瘤]