鼻咽癌^(99m)Tc-HL91乏氧显像与HIF-1α的相关性研究  

Hypoxic Imaging with ^(99m)Tc-HL91 and its Correlation with HIF-1α in NPC

作　　者：吴建国[1] 李金高[1] 

机构地区：[1]江西省肿瘤医院,330029

出　　处：《实用癌症杂志》2010年第4期354-357,共4页The Practical Journal of Cancer

摘　　要：目的研究鼻咽癌99mTc-HL91乏氧显像特征及其与HIF-1α表达的相关性。方法对12例鼻咽癌初诊患者静脉注射99mTc-HL91 740-1110 MBq(20～30 mCi)1 h及4 h后,分别行平面及SPECT断层扫描,应用计算机感兴趣区(ROI),分别勾画各时相的鼻咽肿瘤和颈部淋巴结(T)与对侧相应部位(N)的感兴趣区,计算两者的比值得到T/N值。采用免疫组化法检测肿瘤组织HIF-1α表达水平。结果 12例患者鼻咽部位和(或)颈部淋巴结转移灶均见示踪剂浓集。注射99mTc-HL91示踪剂4 h后病灶显影较1 h明显(P<0.01)。99mTc-HL91断层图像T/N比值始终高于平面图像(P<0.01),而且图像更清晰。示踪剂在鼻咽癌肿瘤部位的浓集与在颈部淋巴结转移灶的浓集无统计学差异(P>0.05)。HIF-1α阳性表达细胞占总细胞数的百分率为39.8%～77.8%(中位数56.4%)。HIF-1α表达与HL91断层图像上鼻咽肿瘤病灶对示踪剂的摄取明显相关(P<0.05),而与颈部淋巴结转移灶对HL91的摄取及平面图像上鼻咽肿瘤病灶的摄取无关(P>0.05)。结论鼻咽癌原发肿瘤病灶和(或)颈部转移淋巴结对乏氧显像剂99mTc-HL91摄取显著,4h后病灶显影较1 h明显。99mTc-HL91断层图像上鼻咽肿瘤病灶对示踪剂的摄取与HIF-1α的表达呈正相关。Objective To study the correlation of 99rnTc-HL91 imaging with HIF-1α expression for nasopharyngeal carcinoma（NPC）.Methods 12 inpatients with pathologically proven NPCs were enrolled into the study before any therapy.Local planar scans and SPECT acquisitions were performed at 1 and 4 hour after intravenous injection of 740-1110 MBq（20～30 mCi）99m↑Tc-HL91.Regions of interest（ROIs） were drawn around the tumor on planar and tomographic 99m↑Tc-HL91 images.The tumor（T） to normal tissue（N） values（T/N） was counted.The highest nuclide accumulation sites of the nasopharyngeal tumors and the cervical node metastases were counted as the tumor（T） values.The expression of HIF-1α was determined by immunohistochemistry.Results Accumulation activity at the nasopharyngeal tumor sites was identified in all patients on planar images and most prominently identified by SPECT slices.Tumor activity is more easily identified at 4 hr rather than at 1 hr postinjection（P〈0.01）.T/N ratios are consistently much higher with 99m↑Tc-HL91 SPECT images than planar images（P〈0.01） in this study.The accumulation of the tracer at the nasopharyngeal tumor sites has no statistical difference with the cervical node metastases（P〉0.05）.HIF-1α expression（39.8%～77.8%,mean：56.4%） was better correlated with the uptake of HL91 at the nasopharyngeal tumor sites on SPECT slices while it wasn＇t correlated with the uptake of HL91 of the nasopharyngeal tumor sites on planar images and the uptake of HL91 of the metastatic cervical nodes.Conclusion 99m↑Tc-HL91 imaging afforded good images to demonstrate the uptake of the tracer at the nasopharyngeal tumor sites and the cervical node metastases.The uptake of HL91 at the nasopharyngeal tumor sites on SPECT slices was better correlated with HIF-1α expression.Both meant hypoxia existed in NPCs.

关 键 词：鼻咽癌 99mTc-HL91乏氧显像 HIF-1Α蛋白 免疫组化 相关性 

分 类 号：R739.63[医药卫生—肿瘤]