2,2′,4,4′-四溴联苯醚(BDE-47)污染沉积物对铜锈环棱螺肝胰脏的SOD、CAT和EROD活性的影响  

Effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47)-contaminated sediments on SOD,CAT,and EROD activities in the hepatopancreas of Bellamya aeruginosa

作　　者：周科[1] 马陶武[1] 朱程[1] 刘佳[1] 彭巾英[1] 吴波[1] 

机构地区：[1]吉首大学生物资源与环境科学学院,吉首416000

出　　处：《环境科学学报》2010年第8期1666-1673,共8页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金项目(No.20677021)~~

摘　　要：以实验室培养的铜锈环棱螺(Bellamya aeroginosa)为受试生物,研究了2,2′,4,4′-四溴联苯醚(BDE-47)污染沉积物对铜锈环棱螺肝胰脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和7-乙氧基-3-异吩唑酮-脱乙基酶(EROD)活性的影响,以揭示BDE-47与这些酶活性之间的剂量-效应和时间-效应关系.结果表明,不同水平BDE-47污染沉积物暴露后,铜锈环棱螺肝胰脏SOD和CAT活性表现出较为明显的剂量或时间依赖性效应,BDE-47可引起铜锈环棱螺肝胰脏的氧化应激,高剂量(≥160ng.g-1)BDE-47的长时间暴露可导致SOD和CAT活性显著下降,提示细胞出现氧化损伤.SOD对BDE-47胁迫的敏感性高于CAT.铜锈环棱螺肝胰脏中SOD和CAT可以作为指示低水平BDE-47污染沉积物胁迫的生物标志物.BDE-47不能诱导铜锈环棱螺肝胰脏EROD活性,但高剂量(≥160ng.g-1)或长时间BDE-47暴露则导致EROD活性显著降低.Bellamya aeruginosa was chosen as a test species to investigate the effects of 2,2＇,4,4＇-tetrabromodiphenyl ether（BDE-47）-contaminated sediments on the activities of superoxide dismutase（SOD）,catalase（CAT）,and 7-ethoxyresorufin O-deethylase（EROD）in the hepatopancreas.The concentration-and time-effect relationships of the three enzymes to BDE-47 exposure are discussed.The hepatopancreatic SOD and CAT activities of Bellamya aeruginosa following exposure to BDE-47-contaminated sediments displayed clear concentration-and time-dependent effect relationships.BDE-47 could cause oxidative stress to the hepatopancreas.Chronic exposure of BDE-47 with high concentration（≥160 ng·g-1）led to significantly decreased SOD and CAT activities,which suggested that hepatopancreas cells were damaged by oxidation.SOD was more sensitive to BDE-47 stress than CAT.The hepatopancreatic SOD and CAT can be used as potentially effective biomarkers indicating the chronic stress of low concentrations of BDE-47 in sediments.BDE-47 did not induce hepatopancreatic EROD activity,rather high concentration BDE-47（≥160 ng·g-1）exposure or chronic BDE-47 exposure significantly reduced EROD activity.

关 键 词：2 2′ 4 4′-四溴联苯醚 沉积物 铜锈环棱螺 肝胰脏 氧化胁迫 7-乙氧基—3-异吩恶唑酮—脱乙基酶 

分 类 号：X171.5[环境科学与工程—环境科学]