Derivation of embryonic stem cells from Brown Norway rats blastocysts  

作　　者：Xiaoyang Zhao Zhuo Lv Lei Liu Liu Wang Man Tong Qi Zhou 

机构地区：[1]State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [2]Graduate School of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Journal of Genetics and Genomics》2010年第7期467-473,共7页遗传学报（英文版）

基　　金：supported in part by the grants from the Chinese National Basic Research Program (No 2007CB948001);the National High Technology R&D Program of China (No 2006AA02A101);Integrated Project funded by the Sixth Framework Programme (FP6) of the European Union (Contract:LSHG-CT-2005-019015)

摘　　要：Knockout Brown Norway （BN） rat could be a useful disease model for human disorders,however,a failure to derive embryonic stem （ES） cells disturbs the further development of the model.In this study,we reported a case of successful derivation of the BN rat ES cells with the derivation efficiency comparable to that of Sprague Dawley （SD） rats.The BN rat ES cells expressed the key transcription factors,and were able to form embryonic bodies （EBs） when being differentiated in vitro.After injecting the BN rat ES cells into the SD rat blastocysts,high-contribution chimeric rats were generated and could survive to their adulthood.Our success in generating pluripotent rat ES cells will benefit the generation of the knockout rats in the future.Knockout Brown Norway （BN） rat could be a useful disease model for human disorders,however,a failure to derive embryonic stem （ES） cells disturbs the further development of the model.In this study,we reported a case of successful derivation of the BN rat ES cells with the derivation efficiency comparable to that of Sprague Dawley （SD） rats.The BN rat ES cells expressed the key transcription factors,and were able to form embryonic bodies （EBs） when being differentiated in vitro.After injecting the BN rat ES cells into the SD rat blastocysts,high-contribution chimeric rats were generated and could survive to their adulthood.Our success in generating pluripotent rat ES cells will benefit the generation of the knockout rats in the future.

关 键 词：Brown Norway （BN） rat embryonic stem cells PLURIPOTENCY chimera rat 

分 类 号：Q813[生物学—生物工程] Q95-331