健胃愈疡颗粒调控热休克蛋白抗大鼠胃溃疡复发的蛋白质组学研究  

Proteome analysis of Jianweiyuyang Granule in the rat model of gastric ulcer recurrence through regulating heat shock proteins

作　　者：申定珠[1] 李家邦[2] 蒋荣鑫[2] 凌江红[2] 

机构地区：[1]上海中医药大学附属曙光医院上海中医药大学肝病研究所,上海201203 [2]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《中国中西医结合消化杂志》2010年第3期168-172,共5页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：湖南省自然科学基金资助项目(04JJ3099)

摘　　要：[目的]分析健胃愈疡颗粒(JWYY)对乙酸性胃溃疡(GU)复发大鼠胃组织蛋白质组表达的影响,寻找JWYY抗GU复发的热休克蛋白(HSP)。[方法]应用固相pH梯度(IPG)双向凝胶电泳技术(2-DE)分离对照组和JWYY组大鼠胃组织的总蛋白,胶体考马斯亮蓝染色后,Image Scanner扫描2-DE获取图像,PDQuest图像分析软件分析比较2-DE图谱并识别2组间差异表达的蛋白质,基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)获得相应的肽质量指纹图谱(PMF),利用Mascot、NCBInr及SWISS-PROT数据库搜索鉴定蛋白质。[结果]JW-YY组与对照组2-DE的平均匹配率为92.3%,12个已鉴定的HSP差异蛋白质中有HSP27、免疫球蛋白重键结合蛋白(GRP78)、HSP70、HSP60,并且以上HSP蛋白在JWYY组大鼠胃组织中表达均上调。[结论]GU复发受多种HSP的调节,调节相关HSP的上增性表达可能即是JWYY抗GU复发的途径之一。[Objective]To investigate heat shock proteins（HSPs）involed in the effect of Jianweryuyang Granule（JWYY） on the expression of proteome in gastric tissue of rats with gastric ulcer recurrence induced by acetic acid.[Methods]The total proteins extracted from gastric tissue in rats with gastric ulcer recurrence were separated by immobilized pH gradient（IPG） two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis（2-DE）.Then proteins underwent,modified Comassie Brilliant Blue staining images,obtained by scanning 2-DE with the Image Scanner apparatus,underwent PDQuest analysis to identify different expressions of proteins between the two groups.Images of peptide mass fingerprinting were obtained with matrix assisted laser desorption ionization time of flight mass spectrometry（MALDI-TOF-MS）,being identified by searching in Mascot,NCBInr and SWISS-PROT databases.[Results]The match rate between the JWYY group and the control group were 92.3%.Of the 12 identified HSPs,HSP27,GRP78,HSP70 and HSP60 expressed more in JWYY-treated group versus the control group.[Conclusion]Gastric ulcer recurrence is regulated by several HSPs,and JWYY may reduce recurrence of gastric ulcer through increasing expressions of correlated HSPs.

关 键 词：胃溃疡 复发 热休克蛋白 蛋白质组 健胃愈疡颗粒 

分 类 号：R573.1[医药卫生—消化系统] Q51[医药卫生—内科学]