P21^(cipl/wafl)基因在C57BL/6N系小鼠腭突发育中对细胞增殖周期的调控作用  

The regulation of P21 cipl/wafl gene on the mesenchymal cell proliferation cycle during palatal process development in C57BL/6N strain mouse

作　　者：李鑫[1] 金岩[1] 董绍忠[1] 朱萧玲[1] 

机构地区：[1]西安第四军医大学口腔医学院病理科

出　　处：《实用口腔医学杂志》1999年第1期57-59,共3页Journal of Practical Stomatology

摘　　要：目的：探讨腭裂形成过程中腭突间充质细胞的增殖周期的变化及其调节基因Ｐ２１ｃｉｐｌ／ｗａｆｌ基因表达的意义。方法：用原位杂交、ＴＵＮＥＬ染色检测两组小鼠腭突间充质细胞增殖周期调控基因Ｐ２１ｃｉｐｌ／ｗａｆｌ基因的表达及细胞程序性死亡的发生。结果：在腭突发育的垂直生长期、上抬期，实验组中ＴＵＮＥＬ阳性指数明显高于对照组（Ｐ＜０．０１）。同时，Ｐ２１ｃｉｐｌ／ｗａｆｌ基因ｍＲＮＡ的转录水平也比同期对照组腭突间质细胞中Ｐ２１ｃｉｐｌ／ｗａｆｌ基因的表达增高（Ｐ＜０．０５）。结论：在腭突发育时期，实验组小鼠腭突间充质未分化细胞增殖周期抑制。Objective: To study the cell cycle in palatal mesenchymal cells during formation of cleft palate and to observe the expression of P21 cipl/wafl gene. Methods: TUNEL staining was used to detect the programmed cell death and in situ hybridization in palatal mesenchyme was applied to study the expression of P21 cipl/wafl gene in the fetus mice with all trans retinoic acid induced cleft palate (experimental group) and in those with normal palate(the control).Results: TUNEL index was higher in the experimental group than in the control in vertical and raising stage of palate development( P <0.01), the level of mRNA transcription of P21 cipl/wafl gene was also heiger in experimental group than in the control ( P <0.05). Conclusion: Cell cycle inhibition of mesenchymal cells in palatine process may result in cleft palate and P21 cipl/wafl overexpression may play an important role in the inhibition.

关 键 词：P21^cip1/waf1 细胞凋亡 鄂突发育 基因调控 

分 类 号：R782.220.2[医药卫生—口腔医学] R329.28[医药卫生—临床医学]