高羊茅FaChit1基因组DNA的克隆及其拷贝数的验定  

Cloning,Characterization of the Full-length Genomic Sequence of Chitinase Gene FaChit1 from Festuca arundinacea and Determination of Gene Copy

作　　者：王健[1,2] 徐子勤[2] 

机构地区：[1]安康学院农学与生命科学学院,陕西安康725000 [2]西北大学西部资源和生物技术教育部重点实验室,西安710069

出　　处：《西北植物学报》2010年第7期1296-1301,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30870194);陕西省自然科学基金(2006C103)

摘　　要：以获得的高羊茅FaChit1基因cDNA设计引物扩增其基因组DNA,序列比对发现该基因内不存在内含子.进一步采用染色体步移方法分离FaChit1基因上游的一段935bp启动子序列以及下游的一段470bp3′非翻译区序列发现,在该启动子区域内不仅含有保守的TATA盒和CAAT盒,而且包含多个潜在的与胁迫应答有关的顺式调控元件,表明该启动子可能是1个多胁迫诱导型启动子.此外,在FaChit13′非翻译区含有真核生物基因中高度保守的特征序列.Southern杂交结果表明,FaChit1在高羊茅基因组中有2个拷贝.To investigate the structure of FaChit1,genomic DNA corresponding to its coding region was cloned and no intron was found.A 5′-flanking region containing 935 bp upstream of the FaChit1 start codon and a 3′-UTR region containing 470 bp were isolated from Festuca arundinacea genomic DNA using genome walking method.Sequence analysis of this FaChit1 promoter fragment revealed that both conserved TATA-box as well as CAAT-box and several potential cis-acting elements associated with stress-related responses were located within the Fachit1 promoter,indicating that this promoter might be multi-stress inducible.Besides,there were some highly conserved special sequences in the 3′-UTR region.Southern blotting revealed that there were two copies of FaChit1 in the F.arundinacea genome.

关 键 词：高羊茅 FaChit1 启动子 拷贝数 

分 类 号：Q789[生物学—分子生物学]