气相色谱-质谱法测定茶饮料中6种利尿剂  

GC-MS Determination of 6 Diuretics in Tea Drink

作　　者：赵海香[1,2] 郭振福[1] 汪丽萍[3] 袁光耀[1] 赵孟彬[2] 邱月明[4] 

机构地区：[1]河北北方学院农林科技学院,张家口075000 [2]北京锦绣大地农业股份有限公司,北京100049 [3]国家粮食局科学研究院,北京100037 [4]中国标准化研究院,北京100088

出　　处：《理化检验（化学分册）》2010年第7期820-824,828,共6页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：北京市海淀区科委企业博士后科研专项(k2008198)

摘　　要：提出了同时测定茶饮料中6种利尿剂的微波辅助衍生化-气相色沓质谱法（选择离子监测模式）[GC-MS（SIM）]和气相色谱-串联质谱（GC-MS／MS）法。样品经乙酸乙酯提取，弗罗里硅土固相萃取柱净化富集后，以碘甲烷、丙酮、碳酸钾为衍生化试剂，采用微波加热使衍生时间缩短为8min。GC-MS（SIM）和GC-MS／MS两种方法的日内和日间相对标准偏差（n=6）均小于10％，但GC-MS／MS法的检出限（3S／N）和测定下限（10S／N）远低于GC-MS（SIM）法的数值。应用GC-MS／MS法测定茶饮料中6种利尿剂，回收率在65．1％～108．5％之间。Methods of GC-MS（SIM） and C-C-MS/MS were applied to simultaneous determination of 6 diuretics in tea drinks with microwave assisted derivatizatiora The sample was extracted with ethyl acetate and purified on Florisil SPE column. Under the microwave-heating condition, the time of derivatization with CH3I, acetone and K2CO3 was shortened to 8 min. It was found that values of intraday and interday RSDfs （n=6） of the method were less than 10%. Values of detection limit （3S/N） and lower limit of determination （10S/N） by GC- MS/MS was far lower than those obtained by GC-MS（SIM）. The method of GC-MS/MS was applied to the analysis of samples of tea drinks, giving values of recovery in the range of 65. 1%-108. 5%.

关 键 词：气相色诤质谱 利尿剂 微波辅助衍生化 茶饮料 

分 类 号：O657.63[理学—分析化学]