胰腺腺泡细胞钙超负荷在诱发大鼠由水肿性向坏死性胰腺炎转变中的作用  

Effects of calcium overload in the conversion of acute edematous pancreatitis to necrotizing pancreatitis in rats

作　　者：蒲青凡[1,2,3] 严律南[1,2,3] 沈骥[1,2,3] 刘占培 谭建三[1,2,3] 左凤琼 吴兆锋[1,2,3] 

机构地区：[1]华西医科大学附属第一医院普外科 [2]四川省攀枝花市攀钢密地医院外科 [3]四川省卫生干部管理学院

出　　处：《中华医学杂志》1999年第2期143-145,共3页National Medical Journal of China

基　　金：国家自然科学基金

摘　　要：目的研究急性水肿性胰腺炎（ＡＥＰ）向坏死性胰腺炎（ＡＮＰ）转变中胰腺腺泡细胞钙超负荷情况。方法将９６只ＳＤ大鼠随机分３组：Ⅰ组３３只（假手术组）。Ⅱ组３３只（ＡＥＰ组）：采用胰管结扎及静脉注射蛙皮素（１００μｇ／ｋｇ）、促胰液素（１０μｇ／ｋｇ）诱发ＡＥＰ。Ⅲ组３０只（ＡＮＰ组）：在ＡＥＰ模型同时静注质量浓度为１０％高分子右旋糖酐（５００ｍｇ／ｋｇ）诱发ＡＮＰ。应用荧光探针Ｆｕｒａ２研究钙超负荷情况。结果ＡＥＰ模型后１，３，６小时腺泡细胞胞浆游离钙离子浓度持续增高，１小时为（１８０±１７）ｎｍｏｌ／Ｌ，６小时为（２１９±１５）ｎｍｏｌ／Ｌ；ＡＮＰ组由１小时（２１３±１９）ｎｍｏｌ／Ｌ，上升为６小时（４６４±２９）ｎｍｏｌ／Ｌ，与假手术组比差异有显著意义（Ｐ＜００５）。而胰腺细胞质膜Ｃａ２＋ＡＴＰ酶活性持续降低，ＡＥＰ组１小时为（５２０±１９）ｎｍｏｌ·ｍｉｎ－１·ｍｇ蛋白－１，９小时降为（３４５±２６）ｎｍｏｌ·ｍｉｎ－１·ｍｇ蛋白－１；ＡＮＰ组１小时为（２９８±０４）ｎｍｏｌ·ｍｉｎ－１·ｍｇ蛋白－１，９小时降为（１８６±０５）ｎｍｏｌ·ｍｉｎ－１·ｍｇ蛋白－１，与假手术组比Ｐ＜０．０?Objective To investigate the potential of pancreatic acinar cell calcium overload in the conversion of acute edematous pancreatitis (AEP) to necrotizing pancreatitis (ANP). Methods Ninety six Sprague Dawley rats were randomized in three experimental groups. Sham operated control (Group Ⅰ) AEP (Group Ⅱ) was induced by pancreatic duct ligation and intravenous injection of bombesin (100 μg/kg) and secretin (10 μg/kg). ANP (Group Ⅲ) was induced same as group Ⅱ but with a large dose of dextran 110, 000(500 mg/kg) intravenously. Pancreatic acinar cell Ca 2+ overload was studied using fluorescent probe Fura2. Cytosolic free Ca 2+ concentration ([Ca 2+ ]i) in isolated pancreatic acinar cells and Ca 2+ ATPase activity in pancreatic cell plasma membranes were determined 1, 3, 6, 9h respectively after treatment. Results The results showed that pancreatic acinar cell [Ca 2+ ]i was elevated at 1h[(213±19) nmol/L, P <0.05] and increased to (464±29) nmol/L at 6h( P <0.05) in rats with ANP, pancreatic cell plasma membrane Ca 2+ ATPase activity decreased significantly from (29.8±0.4)nmol·min -1 ·mgp -1 at 1h to (18 6±0.5) nmol·min -1 ·mgp -1 at 9h in rats with ANP ( P <0.05). Conclusions In ischemia induced conversion of AEP to ANP, there exists Ca 2+ overload in the pancreatic acinar cells. The decreased pancreatic cell plasma membrane Ca 2+ ATPase activity may be an important reason for acinar cell Ca 2+ overload in the development of acute pancreatitis.

关 键 词：胰腺炎 钙 ATP酶 坏死性 胰腺腺泡细胞 

分 类 号：R576.02[医药卫生—消化系统]