采用3,5-二硝基水杨酸比色法测定饲料总淀粉含量  

Determination of the content of total starch in feed by colorimetric method with 3,5-dinitrosalicylic acid

作　　者：马晶晶[1] 王际英[1] 张利民[1] 王世信[1] 黄炳山[1] 孙永智[1] 

机构地区：[1]山东省海洋水产研究所

出　　处：《中国饲料》2010年第14期38-40,共3页China Feed

摘　　要：采用3,5-二硝基水杨酸(DNS)比色法测定饲料样品中总淀粉含量。结果表明,该方法标准曲线在0.2～1.2mg范围内,线性关系良好,回归方程Y=0.7442X-0.0465,相关系数r=0.9998,平均加标回收率为96.8%～98.1%。实验表明,DNS比色法测定总淀粉含量简单易行,且重复性好,可作为饲料中总淀粉含量的常规测定方法。Colorimetric analysis of total starch content in the diet using 3,5-dinitrosalicylic acid（DNS）at the wavelength of 540 nm was clarified in the paper.Results showed that glucose within the levels of 1.2 mg was linearly correlated with absorbency values（Y=0.7442X-0.0465,r=0.9998）,and the average recovery rate ranged from 96.8 % to 98.1 %.It was concluded that the above method could be conventionally used for total starch content determination in feeds.

关 键 词：饲料 总淀粉 3 5-二硝基水杨酸 

分 类 号：S816.17[农业科学—饲料科学]