酶法合成生物柴油工业化研究进展  

Progress on biodiesel production with enzymatic catalysis in China

作　　者：谭天伟[1] 鲁吉珂[1,2] 聂开立[1] 张海霞[1] 邓利[1] 王芳[1] 

机构地区：[1]北京化工大学生命科学与技术学院北京市生物加工过程重点实验室,北京100029 [2]郑州大学生物工程系,郑州450001

出　　处：《生物工程学报》2010年第7期903-906,共4页Chinese Journal of Biotechnology

基　　金：国家重点基础研究发展计划(973计划)(No.2007CB714304);国家自然科学基金(No.20576013);国家高技术研究发展计划(863计划)(Nos.2006AA020203;2009AA03Z232);北京市自然科学基金(No.2071002)资助~~

摘　　要：介绍了北京化工大学近年来酶法合成生物柴油工业化研究的结果。主要内容包括以下几个方面:高产脂肪酶菌株的选育、脂肪酶发酵工艺优化及放大、脂肪酶固定化方法、酶反应器放大、生物柴油分离精制及副产物甘油综合利用。该脂肪酶假丝酵母Candida sp.99-125在5 m3罐发酵活力不低于8 000 IU/mL,然后将该脂肪酶吸附固定在织物膜上并进行表面改性,用于搅拌罐式反应器生产每吨甲酯的需酶量仅为4.2 kg,产品经分离精制调质后,其各项指标完全符合德国生物柴油生产标准。副产物甘油可用于1,3-丙二醇发酵,30 L发酵罐中1,3-丙二醇的产量可达到76.1 g/L。This paper reports the progress of biodiesel production with enzymatic catalysis in Beijing University of Chemical Technology, one of the leaders in biodiesel R ＆ D in China, which includes screening of high-yield lipase production strains, optimization and scale-up of the lipase fermentation process, lipase immobilization, bioreactor development and scale-up, biodiesel separation and purification and the by-product glycerol utilization. Firstly, lipase fermentation was carried out at industrial scale with the 5 m3 stirred tank bioreactor, and the enzyme activity as high as 8 000 IU/mL was achieved by the species Candida sp. 99-125. Then, the lipase was purified and immobilized on textile membranes. Furthermore, biodiesel production was performed in the 5 m3 stirred tank bioreactor with an enzyme dosage as low as 0.42%, and biodiesel that met the German biodiesel standard was produced. And in the meantime, the byproduct glycerol was used for the production of 1,3-propanediol to partly offset the production cost of biodiesel, and 76.1 g/L 1,3-propanediol was obtained in 30 L fermentor with the species Klebsiella pneumoniae.

关 键 词：生物柴油 酶催化转化 脂肪酶固定化 1 3-丙二醇联产 

分 类 号：TE667[石油与天然气工程—油气加工工程]