圆红冬孢酵母两阶段培养法生产微生物油脂  

Microbial lipid production by Rhodosporidium toruloides in a two-stage culture mode

作　　者：林金涛[1,2] 沈宏伟[1] 张泽会[1,2] 胡翠敏[1,2] 靳国杰[1,2] 谭海东[1] 赵宗保[1,3] 

机构地区：[1]中国科学院大连化学物理研究所,大连116023 [2]中国科学院研究生院,北京100049 [3]大连洁净能源国家实验室,筹大连116023

出　　处：《生物工程学报》2010年第7期997-1002,共6页Chinese Journal of Biotechnology

摘　　要：为缩短发酵时间,减少原料消耗,采用细胞增殖和油脂积累分离的两阶段模式,培养圆红冬孢酵母Rhodosporidium toruloides AS 2.1389生产微生物油脂。结果表明,细胞增殖阶段获得的R.toruloides AS 2.1389细胞,重悬接种在葡萄糖溶液中,可快速积累油脂,菌体油脂含量超过自身干重的55%。增殖阶段细胞的菌龄越高,产油能力越强。油脂积累阶段使用高浓度葡萄糖溶液或未灭菌的葡萄糖溶液,油脂合成可以有效进行。油脂中脂肪酸成分以含16和18个碳原子的长链脂肪酸为主,可作为制备生物柴油的新型原料。To shorten the cultivation time and reduce the consumption of raw materials for microbial lipid production, oleaginous yeast Rhodosporidium toruloides AS 2.1389 was cultivated using a two-stage culture mode, in which the cell propagation and lipid accumulation were separated. The yeast cells recovered from the propagation culture were re-suspended in glucose solution for lipid accumulation, through which lipid content over 55% of the dry cell weight was achieved, the longer the propagation stage was, the higher the lipid content. Analysis of the lipid indicated that the long-chain fatty acids with 16 and 18 carbon atoms were major components, suggesting that the lipid can be an alternative feedstock for biodiesel production.

关 键 词：产油酵母 两阶段培养 微生物油脂 生物柴油 

分 类 号：TE667[石油与天然气工程—油气加工工程]