水稻XIOsPR10蛋白的原核表达及其RNase活性研究  

Prokaryotic Expression and RNase Activity Analysis of Rice XIOsPR10

作　　者：郭迟鸣[1] 毛倩[1] 王丽娟[1] 李东霄[1] 杨晓坡[1] 郭立佳[1] 汪卫[1] 李敏[1] 郝国静[1] 陈亮[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,厦门361005

出　　处：《中国生物工程杂志》2010年第7期39-42,共4页China Biotechnology

基　　金：国家"863"计划(2007AA10Z132);国家科技重大专项(2008ZX08001-001;2009ZX08009-045B);教育部科技研究重点项目(01102)资助项目

摘　　要：PR10(病程相关蛋白10)类蛋白与植物的抵御外来病害及系统获得性抗性(SAR)有着紧密联系,而且许多PR10类蛋白都具有RNase活性,并通过这种活性抵抗外源病害。根据获得的XIOsPR10基因,将其构建到pET28a中,通过原核表达及磁珠纯化获得目的蛋白,通过RNA消化实验证实XIOsPR10重组蛋白具有RNase活性,进一步揭示了XIOsPR10蛋白的功能。It has been regarded that PR10s played an important role in systemic acquired resistance （SAR）. Furthermore, many PR10 proteins have been reported to exhibit RNase activity and predicted to be responsible for its antibiotic activity. So the Prokaryotic Expression Vector pET28a-XIOsPR10 was constructed and transferred to BL21（DE3）. The recombinant protein XIOsPR10 was obtained by Prokaryotic expression and purification of magnetic microbeads, and showed the RNase activity.

关 键 词：防御 PR10类蛋白 RNase酶活性 

分 类 号：Q789[生物学—分子生物学]