硫化氢在大鼠肝星状细胞氧应激中对ɑ-SMA与细胞周期影响的实验研究  

Effect of hydrogen sulfide on rat hepatic stellate cells in oxidative stress

作　　者：阳丹才让 邓勇[1] 任利[1] 王聪[1] 樊海宁[1] 

机构地区：[1]青海大学附属医院肝胆胰外科,青海西宁810001

出　　处：《现代生物医学进展》2010年第12期2205-2208,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(No.30960374)

摘　　要：目的:探讨硫化氢在大鼠肝星状细胞氧应激中对α-SMA与细胞周期的影响。方法:将大鼠肝星状细胞株HSC-T6分为8组:空白组(B组,HSC-T6);对照组(C组,B组+500μmol/L Fe-NTA);NaHS组(N1:C组+20μmol/L NaHS;N2:C组+100μmol/L NaHS;N3:C组+200μmol/L NaHS);格列苯脲组(G1:C组+20umol/LGLBN;G2:C组+200umol/LGLBN;G3:C组+700umol/LGLBN)。用免疫组化的方法定性测定α-SMA,用流式细胞仪测定细胞周期。结果:24h的细胞DNA含量S期对照组和空白组有明显差异(41%vs58.6%,P<0.05),给与NaHS后,细胞DNA含量S期N1、N2和N3组与对照组均有明显差异(62.0%,66.8%,72.8%vs58.6%均P<0.05),给与GLBN处理后,细胞DNA含量S期G1、G2和G3组与对照组均有明显差异(38.6%,34.8%,32.5%vs58.6%均P<0.05),各项指标结果与给与NaHS处理后结果相对应,各组与相对应组比较均有明显差异(P<0.05)。24h的免疫组化照片显示对照组和空白组有明显差异,给与GLBN后,G2组与G3组与对照组有明显差异,给与NaHS后,N1,N2,N3组均与对照组,G1,G2,G3组有显著差异。结论:氧应激下硫化氢对HSC细胞具有保护作用,可抑制肝纤维化的发生发展。Obiective：To study the effect of hydrogen sulfide on oxidative stress in rat hepatic stellate cells（HSC） with respect to ɑ-SMAprotein expression level and the change of cell cycle.Methods：HSC-T6 were divided into eight groups：Blank group（B group,HSC-T6）,Control group（C group,B group＋500μmol/L FeNTA）.NaHS groups（N1：C group＋20μmol/LNaHS,N2：C group ＋100μmol/LNaHS,N3：C group ＋200μmol/L NaHS）,GLBN groups（G1：C group ＋20umol/LGLBN;G2：C group ＋200umol/L GLBN;G3：C group＋700umol/L GLBN）.the espression of ɑ-SMA was detected by Immunohistochemistry,the cell cycle was detected by flow cytometry,Results：1.When HSC were incubated with Fe-NTA,Immunohistochemical photographs of ɑ-SMA protein expression level show that there are significant differences between control group and blank group at 24h;At 24 h the cell DNA content S Phase show that there are significant differences between control group and blank group（41%vs58.6%,P0.05）;2.After HSC were treated with NaHS,Immunohistochemical photographs of α-SMA protein expression level show that there are significant differences between control group、GLBN groups and NaHS groups at 24h;at 24 h the cell DNA content S Phase show that there are significant differences between control group and NaHS groups.After HSC were treated with GLBN,Immunohistochemical photographs of ɑ-SMA protein expression level show that the reare significant differences between control group andG2、G3groupsat24h;at 24hthe cell DNA content S Phase show that there are significant differences between control group and GLBN groups.（38.6%,34.8%,32.5% vs58.6%,bothP0.05）.Conclusion：Hydrogen sulfide may be an antioxidants,which can protect the liver and inhibit the development of Hepatic fibrosis.

关 键 词：肝纤维化 肝星状细胞 硫化氢 硫氢化钠 氧应激α-SMA 

分 类 号：Q95-3[生物学—动物学] R575.2[医药卫生—消化系统]