川芎、白芷萃取物下调大鼠硬脑膜COX-2及PGE2的表达  

Effect of Extract of Angelica and Chuanxiong on Expression of Cyclooxygenase-2 and Prostaglandin E2 in Cerebral Meninges of Rats

作　　者：史兆春[1] 徐武[1] 万琪[1] 

机构地区：[1]南京医科大学附属第一医院神经内科,江苏南京210029

出　　处：《现代生物医学进展》2010年第12期2215-2219,共5页Progress in Modern Biomedicine

摘　　要：目的:观察川芎、白芷提取物对实验性偏头痛大鼠模型COX-2及PGE2表达的影响,为都梁软胶囊治疗偏头痛提供实验依据。方法:48只健康成年Sprague-Dawley大鼠随机平均分为3组(每组16只):阳性对照组(M组)、空白对照组(CO组()生理盐水加10%DMSO,1ml/100g灌胃三天);实验组(CBM组()川芎白芷提取物,15mg/kg灌胃三天)。除空白对照组外,各组电刺激三叉神经节30min。刺激完毕取硬脑膜测定待测物质。免疫组化、蛋白质印迹(WB)法观察大鼠脑膜COX-2的表达,酶联免疫吸附法(Elisa)观察脑膜中PGE2含量的变化。结果:与CO组相比,M组、CBM组的脑膜COX-2蛋白表达、阳性颗粒数和PGE2含量显著增加(p<0.05)。与M组相比,CBM组COX-2蛋白表达、阳性颗粒数及PGE2的含量显著降低(p<0.05)。结论:川芎、白芷提取物可以抑制脑膜中COX-2、PGE2表达,推测这可能是川芎白芷控制偏头痛急性发作的重要机制之一。Objective：To study the regulation of COX-2 protein expression and PGE2 concentrations by extract from chuanxiong and baizhi in an animal model of migraine and to provide experimental basis for the treatment of migraine by using Du Liang Capsule（DLC）.Methods：48 healthy adult Sprague-Dawley rats were randomly allocated equally to 3 groups（n = 16 each group）：positive control group（group M） and normal control group（group CO）（they were received the intragastric administration of 1 ml/100g NS and DMSO for three days）;experimental group（group CBM）（they was respectively received the intragastric administration of chuanxiong and baizhi extract 15 mg/kg for three days）.Except group CO,all rats were received electrical stimulation on the trigeminal ganglion for 30 min.After sacrificed,COX-2 was evaluated by western blot and immunohistochemistry,PGE2 was observed by ELISA.Results：Compared to group CO,the expression of COX-2,and release of PGE2 from the dura mater were extremely enhanced（P0.05） in group M and CBM.In contrast to group M,the expression of COX-2 and PGE2 was significantly down-regulated in group CBM（P 0.05）.Conclusion：Regulation of COX-2 and PGE2 in the meningeal blood vessels was assumed to be related with analgesic effect of chuanxiong and baizhi.

关 键 词：实验性偏头痛动物模型 脑膜无菌性炎症 环氧化酶2 前列腺素E2 

分 类 号：Q95-3[生物学—动物学] R747.2[医药卫生—神经病学与精神病学]