急性脑梗死患者血管内皮生长因子与自由基SOD、MDA的临床研究  

A Clinical Study on Vascular Endothelial Growth Factor and Free Radicals SOD,MDA in Patients with Acute Cerebral Infarction

作　　者：何建明[1] 李玉蓉[1] 韦英秀[1] 胡宏波[2] 尹琦[2] 

机构地区：[1]柳州市柳铁中心医院神经内科,广西柳州545007 [2]柳州市柳铁中心医院检验科,广西柳州545007

出　　处：《现代生物医学进展》2010年第12期2295-2298,共4页Progress in Modern Biomedicine

基　　金：广西卫生厅科研课题(Z2009321)

摘　　要：目的:探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)、自由基SOD和MDA在急性脑梗死发病中的作用。方法:分别采用双抗体夹心ELISA法、黄嘌呤氧化法和比色法动态测定60例急性脑梗死患者及正常对照者起病后或入组后第1天、3天、7天、14天外周血清VEGF和SOD、MDA水平,分析其关系。结果:脑梗死组各时间点血清VEGF的水平明显高于正常对照组(P<0.01),至病程第7天达高峰,以后逐渐下降,至14天仍维持较高水平,差异有统计学意义(P<0.01);脑梗死组发病第1天及第3天,SOD水平明显低于对照组,MDA水平明显高于对照组(P<0.01),在第7天及14天,两组比较无显著性差异;患者血清VEGF水平在发病第3、14天随着梗死面积增大及病情严重程度而升高,三组比较有显著性差异(P<0.01),而血清SOD和MDA水平只在发病第3天才与梗死面积及病情严重程度有变化(P<0.01)。结论:VEGF、MDA和SOD共同参与脑梗死急性期血管病理损伤过程,VEGF可作为评估急性脑梗死患者病情轻重和预后的客观指标。Objective：To investigate the effect of VEGF,Free radicals SOD,and MDA in acute cerebral infarction.Methods：Serum VEGF levels,in 60 patients with acute cerebral infaition,were detected by double-antibody sandwich ELISA at the 24h,3d,7d,14d.Plasma serum SOD,MDA levels were determining by Xanthic oxygenation,spectrophotometer at the same time,and 60 healthy individuals were used as control.Results：The level of serum VEGF in cerebral infarction group was significantly higher than that in the normal control group（P0.01） at different times,,which reached the peak at 7th day and,began to decline,maintain a high level untill 14th day,the difference was statistically significant（P0.01）;The SOD levels of cerebral infarction were significantly lower than that in the control group within 24 hours and in the third 3rd days.MDA levels were significantly higher（P0.01）,and at the 7th,14th days,there was no significant difference between the two groups;VEGF levels increased with the increasing of the area of cerebral infarction and the severity of the state of illness at 3rd and 14th days,there was significant difference between the three groups（P0.01）.Serum levels of SOD,MDA changed following infarction area and patient＇s the severity of the state of illness only at the 3rd days（P0.01）.Conclusion：VEGF,SOD and MDA may take part in cerebral vascular pathological damage.VEGF can be used as an important reference index to evaluate the condition and prognosis of acute cerebral infarction.

关 键 词：急性脑梗死 血管内皮细胞生长因子 超过氧化物歧化酶 丙二醛 

分 类 号：R743[医药卫生—神经病学与精神病学]