稻种资源的储藏特性与抗氧化代谢的关系  

Storage longevity and antioxidation metabolism of rice germplasms

作　　者：蒋家月[1,2] 吴跃进[2] 张从合 蒋家平[3] 刘斌美[2] 许学[2] 

机构地区：[1]安徽农业大学茶与食品科技学院,合肥230036 [2]中国科学院等离子体物理研究所,离子束生物工程学重点实验室,合肥230031 [3]安徽荃银高科种业股份有限公司,合肥230031

出　　处：《安徽农业大学学报》2010年第3期387-394,共8页Journal of Anhui Agricultural University

基　　金：中国科学院知识创新工程重要方向项目(kjcx2-yw-n34)资助

摘　　要：对68份不同进化阶段、不同种皮色稻种资源的储藏特性、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、还原型谷胱甘肽(GSH)、花色苷含量、1,2-二苯基苦基苯肼清除率(SFRC)进行测定,研究稻种储藏过程中体内自由基清除率、抗氧化相关酶类和非酶抗氧化物质的变化。结果表明,稻种资源的储藏特性、自由基清除率(SFRC)、抗氧化相关酶类和非酶抗氧化物质存在显著的基因型差异。老化指数与SFRC、花色苷、GSH、POD呈负相关,SFRC与SOD、CAT呈正相关,与种皮中花色苷含量呈极显著正相关(r=0.641**)。随着储藏时间的延长,其体内抗氧化酶(SOD、POD、CAT)活性逐渐降低,GSH、SFRC也呈下降的趋势,而花色苷含量变化不显著。该研究结果为利用稻种资源的有益基因,开展耐储藏专用型水稻品种选育奠定了基础。Relationship between storage longevity and antioxidation metabolism in 68 rice germplasms was studied in this paper.There were significant diversities among varieties in scavenging free radical of DPPH capacity（SFRC）,anthocyanin content,GSH content,superoxide dismutase（SOD） activity,catalase（CAT） activity and peroxidase（POD） activity.Ageing index was negatively related with SFRC,anthocyanin,GSH and POD.SFRC was positively related with SOD and CAT.SFRC was significantly positively related with anthocyanin and the correlation coefficient was 0.641＊＊.Along with the storage,the activities of antioxidative emzymes（SOD,POD,CAT） were depressed gradually.GSH and SFRC were also decreased,while the anthocyanin content was stabile.The results may be important to utilize the availability genes in rice germplasms to breed long longevity rice varieties.

关 键 词：稻种资源 储藏特性 抗氧化 

分 类 号：S511.024[农业科学—作物学]