超滤法提纯万古霉素的应用  

Purification of vancomycin by ultrafiltration

作　　者：王健[1] 张雪霞[1] 王海燕[1] 李晓露[1] 张金娟[1] 张丽[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司微生物药物国家工程研究中心,石家庄050015

出　　处：《生物加工过程》2010年第4期17-21,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家科技支撑计划资助项目(2007BA126B07)

摘　　要：采用Ultra-flo超滤系统提纯未经任何预处理的万古霉素发酵液。结果表明:万古霉素收率由传统工艺(板框加压过滤)的87%提高到94%,滤液的透光度比传统工艺提高了25%;系统平均膜通量可达120L/(m2.h);被污染的膜经清洗后与新膜没有明显差异;合理加水量为投料量的2.5倍。Ultra-flo超滤系统完全能代替传统工艺。Purification vancomycin from fermentation broth by ultrafiltration was investigated.The Ultra-Flo membrane system was used without pretreatment of the fermentation broth.The yield of vancomycin was 87% to 94% and the penetrability of filter liquid enhanced 25% compared with the traditional process.Membrane flux was 120 L/（m2·h）.There was no significant difference between new and contaminated membranes after clearing.The rational quantity of water used in filtering was 2.5 times than that of the original feed.

关 键 词：发酵液 提纯 超滤 万古霉素 

分 类 号：TQ028.8[化学工程]