p53基因和血管生成抑制素基因共转染对胃癌SGC7901细胞凋亡的影响  

Effect of co-transfection of p53 and angiostatin gene on the apoptosis of gastric cancer SG7901 cells

作　　者：陈祥锦[1] 朱月永[2] 胡震霆[2] 张惠灏[1] 翁绳美[3] 庄和珠[1] 

机构地区：[1]福建医科大学附属第一医院肿瘤外科,福州350005 [2]福建医科大学附属第一医院肝病中心,福州350005 [3]福建医科大学药学系,福州350005

出　　处：《肿瘤》2010年第7期577-580,共4页Tumor

基　　金：福建省自然科学基金资助项目(编号:C0310017)

摘　　要：目的:探讨血管生成抑制素(angiostatin,AS)基因和p53基因共转染人胃癌细胞株SG7901后对其凋亡的影响。方法:分别将p53、AS基因以及p53联合AS基因转染SG7901细胞;采用RT-PCR法检测转染后SG7901细胞中目的基因的表达;通过细胞集落形成实验和MTT法观察不同转染对细胞的生长抑制作用;FCM法检测p53基因和AS基因对SGC7901凋亡的影响。结果:经p53或AS基因转染后,SG7901细胞的集落数量及大小均有不同程度的降低;且p53和AS基因具有协同作用,以共转染组降低最为明显,差异有统计学意义(P<0.05)。MTT法检测结果表明,共转染组细胞的生长情况较其他2组单基因转染的细胞更为缓慢(P<0.05)。共转染组凋亡率较AS基因单转染组、p53基因单转染组以及空载体转染组均高,差异有统计学意义(P<0.05)。结论:p53和AS基因均能诱导胃癌细胞SG7901的凋亡,且两者具有协同作用。Objective：To investigate the effects of co-transfection of p53 and angiostatin（AS）gene on the apoptosis of gastric cancer SG7901 cells.Methods：The p53 gene and AS gene were transfected into human gastric cancer cell line SGC7901 separately or in combination.After transfection,RT-PCR was used to detect the expression of the aim genes.Cell clone formation test,MTT assay,and flow cytometry（FCM） were used to observe the effect of p53 gene and AS gene on apoptosis of SGC7901 cells.Results：The number and size of cell clones in p53 or AS gene transfected groups decreased compared with that in untransfected group.P53 gene had synergistic effects with AS gene.The number and size of cell clones decreased the most in co-transfection group.The difference was significant（P0.05）.MTT assay showed that cells grew slower in co-transfection group compared with other two single transfection groups（P0.05）.The apoptosis rate in combined gene transfection group was higher than that in single p53 and AS gene transfection groups and empty vector group.The differences were significant [（33.933±0.306）% vs（18.070±0.305）%,（0.264±0.005）%,（0.890±0.004）%,P0.05].The apoptosis of SG7901 was induced by the two genes.The co-transfection of the combined genes is more powerful than single one.Conclusion：p53 and AS gene could induce the apoptosis of gastric cancer SG7901 cells.They had synergistic effects.

关 键 词：胃肿瘤 基因 p53 血管生成抑制剂 转染 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]