抗人GPC3单克隆抗体在肝脏肿瘤组织学检测中的应用  

Application of anti-GPC3 monoclonal antibody in examination of hepatocellular tumors

作　　者：周赟[1] 谈冶雄[1] 胡栋平[1] 张蓓蓓[1] 王庆[2] 丛文铭[3] 王红阳[1] 

机构地区：[1]第二军医大学东方肝胆外科医院生物信号转导实验室,上海200438 [2]第二军医大学东方肝胆外科医院综合治疗二科,上海200438 [3]第二军医大学东方肝胆外科医院病理科,上海200438

出　　处：《第二军医大学学报》2010年第7期711-714,共4页Academic Journal of Second Military Medical University

基　　金：国家高技术研究发展计划("863计划")(2006AA02A249);国家自然科学基金委重大研究计划培育项目(90713032);国家科技支撑计划课题(2008BAI63B03);全军医学科学技术研究"十一五"面上课题(06MA154)~~

摘　　要：目的应用抗人GPC3单克隆抗体检测肝脏良、恶性肿瘤组织中GPC3蛋白的表达。方法采用自制鼠抗人GPC3单克隆抗体及免疫组织化学技术检测19例正常肝组织、94例肝细胞癌(HCC)组织、20例胆管细胞癌组织、42例肝脏良性肿瘤组织中GPC3蛋白的表达,并分析GPC3蛋白表达水平与HCC患者临床病理参数的关系。结果 GPC3蛋白在肝细胞癌组织中阳性率达到79.8%,而血管瘤旁正常肝组织、胆管细胞癌组织和肝脏良性肿瘤组织中均不表达,差异具有统计学意义(P<0.001)。HCC组织中GPC3蛋白的表达与血清AFP水平(P=0.020)、是否伴有门静脉癌栓(P=0.050)、有无包膜等因素相关(P=0.043)。结论抗人GPC3单克隆抗体可特异性检出肝细胞癌组织GPC3蛋白的表达,值得进一步研究以应用于临床诊治。Objective To evaluate the feasibility of using anti-GPC3 monoclonal antibody in discriminating benign and malignant hepatocellular lesions.Methods The expression of GPC3 was examined by semiquantitative immunohistochemistry assessment in 19 normal control samples,94 hepatocellular carcinoma samples,20 intrahepatic cholangiocarcinoma samples and 42 benign hepatocellular samples.The relation of GPC3 expression profile with the clinicopathological factors of HCC was analyzed.Results GPC3 staining was positive in 75（79.8%）of the 94 HCC samples.And GPC3 expression was not detected in normal liver tissue adjacent to hepatic hemangioma,intrahepatic cholangiocarcinoma,or benign hepatic lesions.GPC3 protein expression in HCCs was correlated with serum alpha-fetoprotein level（P=0.020）,tumor encapsulation（P=0.043）,and portal vein invasion（P=0.050）.Conclusion Anti-human GPC3 mouse monoclonal antibody can specifically detect GPC3 protein expression in hepatocellular carcinomas tissues,which warrants further examination for future clinical application.

关 键 词：磷脂酰肌醇蛋白聚糖类 单克隆抗体 肝肿瘤 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]