维生素C对小鼠系统性白念珠菌感染的影响  

Influence of ascorbic acid supplementation on systemic Candidiasis in mice

作　　者：黄海[1] 王彦[1] 李明邦[1] 李德东[1] 许懿[1] 姜远英[1] 

机构地区：[1]第二军医大学药学院药理学教研室,上海200433

出　　处：《第二军医大学学报》2010年第7期731-733,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30500628;30672626;30825041;30630071);国家重点基础研究发展计划("973计划")(2005CB523105);上海市教育发展基金会晨光计划(2007CG51)~~

摘　　要：目的观察维生素C对系统性白念珠菌感染病情的影响。方法 50只ICR小鼠随机分为5组(n=10),通过尾静脉注射白念珠菌菌液建立系统性白念珠菌感染小鼠模型。维生素C通过灌胃或腹腔注射给药,剂量为50mg/kg或500mg/kg;考察小鼠生存时间;检测肾脏载菌量;制作肾脏组织病理学标本评估真菌感染程度。结果补充维生素C后,各实验组与空白组中位生存时间均为4～5d,各组间无明显差异;补充维生素C后,实验组lgCFU/g分别为8.342、8.412、7.350、7.764,与空白组lgCFU/g7.134比较,肾脏载菌量增加(P<0.05)。肾脏组织病理学结果显示受检小鼠均有肾脓肿及肾小管上皮变性(肾病)损害,损害程度没有明显差异,表明维生素C不能缓解白念珠菌感染的病情。结论补充维生素C对小鼠系统性白念珠菌感染病情没有改善作用,甚至可能导致病情恶化。Objective To examine the influence of ascorbic acid supplementation on mouse model of Candida albicans infection.Methods Mouse models of systemic Candida albicans infection were established by the tail vein injection of Candida albicans suspension and were randomly divided into five groups（n=10）.Ascorbic acid was administered by gavage or intraperitoneal injection at a dose of 50 mg/kg or 500 mg/kg.The survival time and renal fungal burden of mice were observed.Renal histopathology specimens were stained by H-E and PAS staining to assess kidney damage.Results The median survival periods of animals in both ascorbic acid supplementation group and blank control group were 4-5 days.The lgCFU/g values were 8.342,8.412,7.350,and 7.764 in experimental groups and 7.134 in the blank control group.Supplementation of ascorbic acid had significant influence on renal fungal burden（P0.05）.Kidney histopathology examination showed renal abscess and renal tubule disorders in mice in all groups,indicating that ascorbic acid supplementation did not alleviate Candida albicans infections.Conclusion Ascorbic acid supplementation can not improve the condition of systemic Candida albicans infection in mice,and can even exacerbate the condition.

关 键 词：抗坏血酸 白念珠菌 系统性真菌感染 

分 类 号：R519.3[医药卫生—内科学]