不同月龄和血脂水平ApoE基因敲除小鼠体内类固醇激素合成急性调节蛋白的表达(英文)  

Expression of steroidogenic acute regulatory protein following with age and serum lipids levels in apolipoprotein E-deficient mice

作　　者：宁艳霞[1] 江一峰[1] 徐晨[1] 赵凤娣[1] 殷莲华[1] 

机构地区：[1]复旦大学上海医学院生理与病理生理学系,上海200032

出　　处：《复旦学报（医学版）》2010年第4期422-429,共8页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金项目(30900716,30871021)

摘　　要：目的检测不同月龄和不同血脂水平载脂蛋白E敲除小鼠体内类固醇激素合成急性调节蛋白(steroidogenic acute regulatory protein,StAR)的表达水平。方法实验分别选取雄性和雌性1天龄新生鼠、1月龄、3月龄及5月龄载脂蛋白E敲除小鼠(apoE-/-)及对照C57BL/6J小鼠,每组6只,共16组。利用试剂盒检测不同年龄小鼠的血脂水平。利用实时定量RT-PCR及Western blot方法检测不同年龄和血脂水平下,小鼠肝脏中StAR基因和蛋白的表达。结果相比同年龄同性别的正常对照小鼠,载脂蛋白E敲除小鼠血清中含较高水平的低密度脂蛋白(LDL)和较低水平的高密度脂蛋白(HDL)。在对照小鼠中,StAR基因和蛋白表达水平随月龄增加逐渐下降;但是在载脂蛋白E敲除小鼠体内,因为血脂水平的提高,StAR基因和蛋白表达水平呈现先增高后降低的趋势。结论StAR通过调节脂质代谢,可作为一个有效调节动脉粥样硬化以及其他心血管疾病的调节因子。Objective To detect steroidogenic acute regulatory protein（StAR） expression in apolipoprotein E-deficient mice at different ages and serum lipid levels. Methods Nighty-six C57BL/6J and apoE-/-mice were enrolled,which were divided into 16 groups with 6 mice per group according to age（1 day,1,3,5 months）,sex and genotype（C57BL/6J and apoE-/-）.The serum lipid levels in C57BL/6J and apoE-/-mice were detected by commercial kits.StAR mRNA and protein expressions in liver were detected by Real-time PCR and Western blot respectively. Results ApoE-/-mice had higher LDL-cholesterol and lower HDL-cholesterol compared with C57BL/6J mice of the same age and sex.StAR mRNA and protein expressions were decreased following with aging in C57BL/6J mice.However,in apoE-/-mice with higher lipid levels,StAR mRNA and protein expressions were changed with the lipid levels other than ages.StAR mRNA and protein increased in the early stage,and then decreased with the increasement of lipids levels. Conclusions StAR could affect lipids levels and may be an effective regulator for atherosclerosis and other cardiovascular diseases.

关 键 词：类固醇激素合成急性调节蛋白 胆固醇 载脂蛋白E敲除小鼠 C57BL/6J小鼠 氧化胆固醇 

分 类 号：R363[医药卫生—病理学] R54[医药卫生—基础医学]