雌二醇与姜黄素单用或联用对海人酸杏仁核点燃大鼠海马GAD_(67)表达的影响  

Effects of estradiol or/and curcumin on GAD_(67) expression in the hippocampus of epileptic rat model induced by kainic acid introamgydale injection

作　　者：彭伟锋[1] 郭曦[2] 汪昕[1] 杜鹏[1] 

机构地区：[1]复旦大学附属中山医院神经内科,上海200032 [2]复旦大学附属中山医院分部内科,上海200052

出　　处：《复旦学报（医学版）》2010年第4期455-458,共4页Fudan University Journal of Medical Sciences

摘　　要：目的观察腹腔注射雌二醇(estradiol,E)或/和姜黄素(curcumin,C)后海人酸(kainic acid,KA)杏仁核点燃大鼠海马谷氨酸脱羧酶(glutamate decarboxylase,GAD)的亚型GAD67表达的变化,探讨在姜黄素干预下雌二醇对GAD67表达的影响。方法将32只去势术后的雌性SD大鼠分为E+KA组、C+KA组、EC+KA组和KA组,分别给予腹腔注射雌二醇或/和姜黄素,5天后均制备成KA杏仁核点燃的癫大鼠模型,采用免疫组化方法检测大鼠海马中GAD67表达的变化。结果统计KA注射对侧(左侧)大鼠海马中GAD67免疫阳性细胞的表达数。在CA1区,C+KA组GAD67的表达与KA组(P<0.01)、E+KA组(P<0.05)相比均明显增多,它在EC+KA组的表达较KA组(P<0.01)也明显增多,差异有统计学意义。在CA3和DG区,C+KA组GAD67的表达与KA组(P<0.01)、E+KA组(CA3区:P<0.01,DG区:P<0.05)、EC+KA组(P<0.05)相比均明显增多。而且在DG区,它在E+KA组的表达较KA组(P<0.05)也有明显增多。结论雌二醇或姜黄素单用时均可以上调KA杏仁核点燃大鼠海马中GAD67的表达,以姜黄素的上调作用更为明显。姜黄素干预后,雌二醇对GAD67的表达无明显影响,但两者联用时仍可使CA1区GAD67的表达明显增多。Objective To observe the changes of GAD67 expression in the hippocampus of epileptic rat model induced by kainic acid（KA） introamgydale injection when adding estradiol（E） or/and curcumin（C） by intraperitoneal injection,and to explore the effects of estradiol on GAD67 expression while interfered with curcumin. Methods Thirty-two ovariotomized female rats were divided into the following groups： E＋KA group,C＋KA group,EC（estradiol and curcumin）＋KA group,and KA group.Rats were given estradiol or/and curcumin by intraperitoneal injection respectively,and epilepsy was induced by microinjection of KA into the amygdala five days later.Immunohistochemical method was used to measure GAD67 expression in the hippocampi of epileptic rats of all groups. Results The number of cells with positive GAD67 expression was counted in the left hippocampi,the opposite site to KA injection.In CA1 region,the number of cells with positive expression of GAD67 in C＋KA group was greater than that in KA group（P0.01） and E＋KA group（P0.05）,it was also greater in EC＋KA group than in KA group（P0.05）.In CA3 and DG region,the number of cells with positive GAD67 expression in C＋KA group was greater than in KA group,E＋KA group（CA3 region： P0.01,DG region： P0.05） and EC＋KA group（P0.05）.In DG region,higher positive cells with GAD67 expression in E＋KA group than in KA group was also found. Conclusions GAD67 expression in the hippocampi of epileptic rats induced by KA introamgydale injection is upregulated by estradiol or curcumin respectively,especially more obvious by curcumin.After interfered with curcumin,the changes of effects of estradiol on GAD67 expression are not significant,but there are still more positive cells with GAD67 expression in CA1 region by the joint action of estradiol and curcumin.

关 键 词：雌二醇 姜黄素 癫 GAD67 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]