纵隔原发性大B细胞性淋巴瘤免疫组化和DNA倍体研究  

Immunohistochemistry and DNA ploidy of primary large B cell lymphoma in mediastina

作　　者：吕翔[1,2] 孟凡青[1,2] 周祀侨 密方元[1,2] 

机构地区：[1]南京大学医学院附属鼓楼医院病理科 [2]南京八一医院病理科

出　　处：《临床与实验病理学杂志》1999年第1期12-14,共3页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家教委留学回国人员科研启动基金

摘　　要：目的：研究纵隔原发性大Ｂ细胞性淋巴瘤的临床病理、免疫组化及ＤＮＡ倍体的表现。方法：采用常规石蜡切片、ＨＥ染色及免疫组化染色ＡＢＣ法。另对６μｍ厚石蜡切片采用Ｆｅｕｌｇｅｎ染色，应用细胞影像仪对肿瘤细胞核的ＤＮＡ倍体进行测定。结果：肿瘤细胞特点为瘤细胞较大，呈弥漫分布，其间可见宽窄不一、成束或带状的纤维结缔组织穿插分割。免疫组化染色瘤细胞表达ＬＣＡ及Ｌ２６阳性，ＩｇＡ及κ弱阳性。经细胞影像仪测定瘤细胞ＤＮＡ呈高异倍体，ＤＮＡ质量的分布呈高度异质性。结论：来源于胸腺Ｂ淋巴细胞。肿瘤细胞ＤＮＡ倍体测定结果对于患者预后的判断可能具有非常重要的意义。Purpose To investigate the characteristics of clinicopathology, immunohistochemistry and the DNA ploidy measurement for the primary large B cell lymphoma in mediastina. Methods The routine paraffin slides were cut and stained with HE as well as ABC immunohistochemical technique. In addition, the 6 μm thick paraffin slides were cut and stained by Feulgen method. Then the DNA ploidy of tumor cells were measured with image cytometer. Results The morphological characteristics of tumor cells were that the sheets of diffusely distributed large lymphocytes were penetrated by bundles of fibroconnective tissue. In immunohistochemically stained slides, the tumor cells were LCA and L 26 positive, IgA and κ weakly positive. The DNA ploidy measurement results showed that the tumor cells were high aneuploidy and the distribution of DNA contents were highly heterogeneous. Conclusion The neoplasm may originate from the B lymphocyte in thymus. The result of the DNA ploidy measurement of the tumor cells suggests that it might have very great significance for the prediction of the prognosis of the patient.

关 键 词：纵隔肿瘤 淋巴瘤 DNA倍体 B细胞 

分 类 号：R734.502[医药卫生—肿瘤] R733.402[医药卫生—临床医学]