pEGFP-N1/VEGF真核表达质粒的构建与鉴定  

Construction and identification of eukaryotic expression vector pEGFP-N1/VEGF

作　　者：张明磊[1] 常非[1] 高忠礼[1] 柳扬[1] 刘光耀[1] 

机构地区：[1]吉林大学中日联谊医院骨科,吉林长春130033

出　　处：《中国实验诊断学》2010年第7期980-982,共3页Chinese Journal of Laboratory Diagnosis

基　　金：高等学校博士点专项科研基金(编号:20060183050)

摘　　要：目的构建pEGFP-N1/VEGF真核表达质粒,并进行鉴定。方法利用RT-PCR方法从组织中提取VEGF的基因片段,与pMD18-T载体连接,构建pMD18-T/VEGF重组质粒,酶切后与pEGFP-N1真核表达载体连接,构建pEGFP-N1/VEGF真核表达质粒,进行测序、酶切鉴定,表明质粒构建成功。结果本实验成功构建了pEGFP-N1/VEGF真核表达质粒。结论为进一步研究利用VEGF基因修饰骨组织工程骨,促进血管再生提供实验基础。Objective To Construct a eukaryotic expression vector carrying VEGF gene.Methods VEGF gene was cloned from tissue by using RT-PCR method and was inserted into pMD18-T to constrcuct pMD18-T/VEGF plasmid,and to construct pEGFP-N1/VEGF plasmid by using restriction enzyme.To identify the plasmid by sequencing and restriction enzyme.Results The eukaryotic expression vector pEGFP-N1/VEGF was constructed and identified.Conclusion It provides the base of engineering bone modified by VEGF gene,revived the blood vessel.

关 键 词：PEGFP-N1 VEGF 基因 

分 类 号：R363[医药卫生—病理学]