Lewis肺癌浸润淋巴细胞的进一步纯化及体外抗癌特点的研究  

A Simple Approach to Purification of Tumor Infiltrating Lymphocytes From Lewis Lung Cancer and It Nonspecific Antitumor Effect in Vitro

作　　者：唐华[1,2] 陈德基[1,2] 刘铭球 刘晓翌 徐惠琴 周洁 张华亭 

机构地区：[1]河南省人民医院病理科 [2]解放军广州医学高等专科学校

出　　处：《湖北医科大学学报》1999年第1期16-18,共3页

基　　金：湖北省教委重点科研基金

摘　　要：为探索进一步纯化肿瘤浸润淋巴细胞（ＴＩＬ）的方法及其体外抗瘤作用特点，为临床应用ＴＩＬ治疗肺癌提供依据，用不连续密度梯度离心法从接种于Ｃ５７ＢＬ／６小鼠体内的Ｌｅｗｉｓ肺癌（ＬＬＣ）瘤体中分离提取ＴＩＬ，过夜贴壁，剔除贴壁细胞后，可明显提高ＴＩＬ的纯度。纯化的ＴＩＬ在含１０００Ｕ／ｍｌＩＬ２的完全培养基中继续培养。免疫荧光表型分析发现，ＴＩＬ中ＣＤ＋４、ＣＤ＋８初为０９１±０１２，培养后为０８３±００７，体外ＴＩＬ对ＮＫ敏感的Ｌ９２９细胞有一定的杀伤作用，效靶比为２００∶１和１００∶１时的杀伤率高于５０∶１（Ｐ＜００５），表明ＬＬＣ浸润淋巴细胞体外的杀伤活性具有一定的非特异性。Tumaor infiltrating lymphocytes (TILs) isolated from Lewis lung cancer(LLC) implanted in C57BL/6 mice by discontinuous density gradients centrifugation were investigated for cytotoxic capacity and analyzed for their phenotypes by indirect immunofluorescence.It was found that the purity of TILs can be significantly increased after the TILs suspension was cultured overnight to get rid of the adherent cells.It was also found that the ratio of CD + 4/CD + 8 was 0.83 after having been cultured in the presence of IL 2 in vitro,while it was 0.91 in the fresh ones.TILs can kill NK sensitive cell line L929 and the lytic activity of TILs was significantly higher when the effect target ratio was 200∶1,100∶1 than that of 50∶1( P <0.05),which suggested that cultured TILs had NK like activity.

关 键 词：肺肿瘤 淋巴细胞 肿瘤浸润 LEWIS肺癌 

分 类 号：R734.2[医药卫生—肿瘤]