丁基苯酞对局灶性脑缺血大鼠软脑膜微循环障碍的影响  

EFFECTS OF 3 n BUTYLPHTHALIDE ON PIAL ARTERIOLES IN FOCAL CEREBRAL ISCHEMIA RATS

作　　者：徐皓亮[1] 冯亦璞[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所

出　　处：《药学学报》1999年第3期172-175,共4页Acta Pharmaceutica Sinica

基　　金：国家科委1035工程重大项目基金;国家自然科学基金

摘　　要：目的：观察消旋、左旋及右旋丁基苯酞（ｄｌ，ｌ，ｄ３ｎｂｕｔｙｌｐｈｔｈａｌｉｄｅ，ｄｌ，ｌ，ｄＮＢＰ）对局灶性脑缺血大鼠软脑膜微循环障碍的影响。方法：用插线法造成大鼠局灶性脑缺血模型，并用体外显微摄像技术及微循环图象处理系统观察大鼠软脑膜微动脉管径及红细胞流速的变化。结果：ｄｌ，ｌ和ｄＮＢＰ对正常大鼠脑微动脉管径无明显影响，ＭＣＡＯ术前１ｈ预防给药，ｄｌ，ｌＮＢＰ和尼莫地平可明显增加局灶性脑缺血大鼠软脑膜微动脉管径及血流速度，而ｄＮＢＰ则加重软脑膜微循环障碍。ＭＣＡＯ术后２０ｍｉｎ治疗给药，ｄｌ和ｌＮＢＰ仍可明显逆转局灶性脑缺血大鼠软脑膜微循环障碍，而ｄＮＢＰ及尼莫地平作用不明显。结论：改善脑微循环状态是ｄｌ和ｌＮＢＰ发挥抗脑缺血作用的重要药理机制之一。AIM: To study the effects of dl , l and d 3 n butylphthalide(NBP) on pial arteriole diameter(AD) and blood flow velocity(BFV) in focal ischemia rats. METHODS: Urethane anesthetized rats were instrumented for monitoring pial AD and BFV in the cranial window preparation. The effects of dl , l , d NBP on AD and BFV were investigated in these left middle cerebral artery occluded(L MCAO) rats by using the method of acute cranial window technique under in vitro videomicroscope. dl , d , l NBP(25 mg·kg -1 ip) and nimodipine(0 3 mg·kg -1 ) were administrated systemically 20 min after MCAO or 1 h before MCAO. RESULTS: In the vehicle group, MCAO induced a significant decrease in BFV and AD, the levels of BFV and AD were reduced to 18 3% and 52% compared with the preischemia baseline values. In the pretreatment groups, no change in pial AD was found after dl , l , d NBP administration in normal animals, and a rapid and marked decrease in BFV and AD of the selected pail artery was observed within 5 minutes after MCAO. The decreased level of AD and BFV recovered quickly after MCAO in the dl , l NBP and nimodipine groups, while the dysfunction of microcirculation was exacerbated by d NBP. In the post treatment groups, dl NBP(12 5, 25 mg·kg -1 ip) induced dilation of the pial arterioles and the increase in BFV was in dose dependent manner. The pial arteriolar response to MCAO was not affected by d NBP and nimodipine. CONCLUSION: These data suggest that the improving effects of dl and l NBP on microcirculation dysfunction during ischemia may play an important role in their protective effects against focal cerebral ischemia injury. l and d NBP showed counteractive effects on pial AD and BFV in MCAO rats indicating that NBP has stereoselective character on its protective action against cerebral ischemia injury.

关 键 词：丁基苯酞 局灶性 脑缺血 软脑膜 微循环障碍 

分 类 号：R743.31[医药卫生—神经病学与精神病学]