基于静电沉积壳聚糖铂纳米颗粒复合膜构建葡萄糖生物传感器  

Electrodeposition of Chitosan and Platinum Nanoparticles for Fabrication of an Amperometric Glucose Biosensor

作　　者：范增杰[1] 马莉萍[1] 李云霞[1] 刘国汉[1] 李工农[1] 韩根亮[1] 

机构地区：[1]甘肃省科学院传感技术研究所,兰州730000

出　　处：《传感技术学报》2010年第6期764-767,共4页Chinese Journal of Sensors and Actuators

基　　金：甘肃省科技支撑计划资助项目(0708GKCA062)

摘　　要：利用电沉积方法对壳聚糖/铂纳米颗粒复合膜进行组装,以戊二醛作为交联剂固定葡萄糖氧化酶,构建葡萄糖生物传感器。实验结果表明:制得的葡萄糖生物传感器响应时间仅为8 s,线性测量范围为1×10-5～1×10-3mol/L,检测限为1.4×10-5mol/L(S/N=3.0)。该传感器灵敏度高,稳定性好。A composite film via deposition of chitosan/platinum nanoparticles was constructed on a gold electrode using electrichemical methods.Glucose biosensor was formed with glucose oxidase immobilized in composite immobilization membrane matrix,which was composed of chitosan and platinum nanoparticles by using glutaraldehyde as a cross-linking agent.The response time of biosensor is only about 8s,with a linear range from 1×10-5 to 1×10-3mol/L and a detection limit of 1.4×10-5mol/L（S/N =3.0）.Meanwhile,the biosensor has higher sensitivity and longtime stability.

关 键 词：生物传感器 固定化酶 葡萄糖氧化酶 壳聚糖 铂纳米颗粒 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]