肿瘤坏死因子α真核表达载体的构建  

Construction of hTNF -α expression vector

作　　者：王言[1] 刘馨[1] Wang Yan ,Liu Xin. （ Tumor department of the first affiliated hospital of Liaoning medical university, Jinzhou 121001 China）

机构地区：[1]辽宁医学院附属第一医院,辽宁锦州121000

出　　处：《中国保健》2009年第16期686-688,共3页

摘　　要：目的：克隆人肿瘤坏死因子α（TNF-α），构建真核表达载体pEGFP—C1／hTNF-α。方法：以总RNA为模板，应用RT—PCR方法扩增TNF-α上游序列长约702bp的片段，克隆入绿色荧光蛋白GFP的基因下游，测序鉴定。结果：DNA测序结果与GeneBank中TNF-αmRNA序列（NM-000594）完全一致，片段长度为702bp。结论：人肿瘤坏死因子的真核表达载体pEGFP—C1／hTNF-α构建成功，为TNF-α功能的进一步研究奠定了基础。Objective： To clone DNA sequence of human tumor necrosis factor -α （ TNF -α）, and to construct eukaryotic expression plasmid of pEGFP - C1/hTNF -α. Methods ：702 bp hTERT promoter were amplified with reverse transcriptase polymerase chain reaction （ RT - PCR） method,utilizing mRNA as template. The TNF-α was inserted into pEGFP- C1 to reconstruct a recombinant plasmid named pEGFP- C1/hTNF-α. And the sequence was detected. Results： DNA sequencing showed a same sequence as registered in Gene Bank （ NM -000594）. The Sequence contains 702 bp. Conclusion ：The construction and the expression of eukaryotic plasmid pEGFP - C1/hTNF -α have been achieved successfully. The research paved the way for the function researchof TNF -α.

关 键 词：人肿瘤坏死因子Α 荧光蛋白 肿瘤 

分 类 号：R730.23[医药卫生—肿瘤;医药卫生—临床医学]