平调饮对原发性肝癌AFP mRNA、GPC-3 mRNA影响的研究  

Study on influence of Pingtiaoyin on expression of AFP mRNA and GPC-3 mRNA in patient with hepatic cell carcinoma

作　　者：宋爱英[1] 孙轶男[1] 刘鲲[1] 王赫 陈端洪[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江省肿瘤医院,黑龙江哈尔滨150040

出　　处：《现代中西医结合杂志》2010年第24期3002-3003,3087,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：黑龙江省科学技术计划攻关项目(GC07C35104)

摘　　要：目的探讨平调饮对原发性肝癌患者AFP mRNA、GPC-3 mRNA表达的影响。方法抽取60例肝癌患者的抗凝血液,配对法随机分为试验组和对照组各30例。采用Tr-Izol一步法提取总RNA,用逆转录巢式聚合酶链反应(RT-nested PCR)技术检测治疗前后试验组及对照组患者AFP mRNA、GPC-3 mRNA的表达情况。结果治疗后试验组及对照组AFP mRNA、GPC-3 mRNA表达均显著下降,在降低AFP mRNA表达方面试验组与对照组无显著性差异,降低GPC-3 mRNA表达方面试验组明显优于对照组(P<0.05)。结论平调饮能有效抑制人血液中AFP mRNA、GPC-3 mRNA的高表达,在降低GPC-3 mRNA表达方面效果优于华蟾素。其治疗原发性肝癌机制与临床应用价值值得进一步研究。Objective It is to approach the influence of Pingtiaoying on expression of AFP mRNA and GPC-3 mRNA in patients with hepatic cell carcinoma（HCC）.Methods 60 patients with HCC were randomly divided into test group（n=30） and control group（n=30）.Total RNA was extracted from HCC peripheral blood samples collected after operation,and nested reverse transcription polymerase chain reaction（RT-nested PCR） assay was performed to determine the expression of AFP mRNA and GPC-3 mRNA.Results The expression of AFP mRNA and GPC-3 mRNA were markedly decreased after treatment.There was no significant difference between the two groups in the decreasing expression of AFP mRNA,but the effect to decrease the expression of GPC-3 mRNA in test group was better than that in control group（P〈0.05）.Conclusion Pingtiaoyin can effectively suppress the expression of AFP mRNA and GPC-3 mRNA in peripheral blood,it decreases the expression of GPC-3 mRNA more than Huachansu.The therapeutic mechanism and clinical application of Pingtiaoyin on patients with HCC deserve further study.

关 键 词：原发性肝癌 平调饮 GPC-3 MRNA AFP MRNA 

分 类 号：R735.7[医药卫生—肿瘤]