薄层色谱法在异甘草素合成中的应用  

Application of thin-layer chromatography in the synthesis of isoliquiritigenin

作　　者：王建国[1,2] 伍林[1] 钟婵娟[2] 刘建华[3] 齐小燕[1] 周锦霞[1] 

机构地区：[1]武汉科技大学化学工程与技术学院,湖北武汉430081 [2]九江学院化学化工学院,江西九江332005 [3]浙江工业大学化学工程与材料科学学院,浙江杭州310032

出　　处：《化学试剂》2010年第7期643-645,648,共4页Chemical Reagents

基　　金：江西省教育厅科技项目(GJJ08433);江西省科技厅科技支撑项目(2008ZD06100)

摘　　要：研究出了一种适合对羟基苯甲醛和2,4-二羟基苯乙酮在碱性条件下合成异甘草素的薄层色谱分析体系。该体系的显色剂为铁氰化钾-三氯化铁试剂;展开剂为乙酸乙酯-苯,体积比V(乙酸乙酯)∶V(苯)=1∶2。展开后2,4-二羟基苯乙酮、对羟基苯甲醛和异甘草素的Rf值分别为0.67、0.58和0.38,3种化合物能有效地被分离。An analytical system of thin-layer chromatography was developed for the synthesis of isoliquiritigenin from p-hydroxybenzaldehyde and 2,4-dihydroxyacetophenone in an alkaline medium.In this system,FeCl3-K3Fe（CN）6 was used as the visualizing reagent.The developing solvent was an ethyl acetate/benzene mixture with a ratio of V（ethyl acetate）∶V（benzene）=1∶2.After development,the Rf values of 2,4-dihydroxyacetophenone,p-hydroxybenzaldehyde,and isoliquiritigenin were 0.67,0.58,and 0.38,respectively.The three compounds could be effectively separated.

关 键 词：薄层色谱法 异甘草素 合成 

分 类 号：O658.1[理学—分析化学]