链脲菌素诱导糖尿病性勃起功能障碍大鼠模型的制备  

The establishment of diabetes mellitus rats model with erectile dysfunction induced by streptozocin

作　　者：苏宏伟[1] 李向东[1] 李凤岐[1] 王哲[1] 陈怀安[1] 

机构地区：[1]河北北方学院附属第一医院,075000

出　　处：《山西医药杂志（上半月）》2010年第8期704-706,共3页Shanxi Medical Journal

基　　金：河北省张家口市科委科学技术研究与发展指导计划项目(0921066D)

摘　　要：目的通过腹腔注射链脲菌素制备糖尿病性勃起功能障碍(DMED)大鼠模型,为研究DMED提供实验动物模型。方法 Wistar大鼠120只,随机分为Ⅰ、Ⅱ、Ⅲ3组,每组分为糖尿病组(DM)20只,对照组(NDM)20只,DM组腹腔注射链脲菌素(60mg/kg)建立DM动物模型后,注射阿朴吗啡(80μg/kg)观察8、12、16周大鼠阴茎勃起情况,制备DMED大鼠模型。结果 DM组大鼠成模49只,8、12、16周分别为17只、17只、15只,注射阿朴吗啡后勃起次数分别为1.0±0.0、1.0±0.0、1.0±0.0,勃起率分别为35%、24%、13%,ED发生率分别为65%、77%、87%;NDM组60只,分别为2.2±0.8、2.3±0.8、2.0±0.7,勃起率均为100%,ED发生率为0。两组相比差异有统计学意义(P<0.01)。结论链脲菌素诱导DMED大鼠模型方法可行,可靠。Objective To establish suitable diabetic rats models with erectile dysfunction（ED）.Methods One hundred and twenty Wistar rats were divided into two groups,the DM groups and the control groups.DM groups were injected intraperitoneally with streptozocin（STZ）（60 mg/kg）to make experimental models of DM.Injected with apomorphine（80 μg/kg）,penile erection of the rat models with DM and the controls were observed and noted on the 8th,12th and 16th week from the 4th day of STZ injection.Results The DM ED rat model was established after 8,12 and 16 weeks,the frequency of penis erection in diabetic group was 1.0±0.0,1.0±0.0,1.0±0.0,with which was 2.2±0.8,2.3±0.8,2.0±0.7 in the control groups;the rate of penis erection in diabetic group was only 35%,24%,13% respectively,which was much lower than that 100% in the control groups（P0.01）.Conclusion The establishment of DM ED rats models which was induced by STZ injected intraperitoneally is feasible and reliable.

关 键 词：糖尿病 实验性 阳痿 大鼠 Wistar 

分 类 号：R-332[医药卫生]