脑梗死人群NOS1基因多态性与脂代谢的相关性  

A population-based study of neuronal nitric oxide synthase (NOS1) gene polymorphism and lipid metabolism in cerebral infarction for Chinese Han

作　　者：高鹏[1] 吴江[2] 杜丹华[2] 李蕴博[3] 赵节绪[2] 胡林森[2] 

机构地区：[1]吉林大学第一医院内分泌科,吉林长春130021 [2]吉林大学第一医院神经内科,吉林长春130021 [3]吉林大学第一医院神经外科,吉林长春130021

出　　处：《中风与神经疾病杂志》2010年第6期489-491,共3页Journal of Apoplexy and Nervous Diseases

基　　金：卫生部重点项目(2007);国家自然科学基金青年基金项目(30800337);吉林省科技厅青年基金项目(No.20080161);吉林省卫生厅项目(2008P008)

摘　　要：目的探讨脑梗死患者神经元型一氧化氮合酶基因(NOS1)多态性与脂代谢的相关性。方法本研究共纳入605例脑梗死患者。以位于NOS1基因的rs9658281和rs2682820位点为遗传标记,采用聚合酶链式反应-限制性片断长度多态性(PCR-RFLP)技术检测NOS1基因的基因型。应用氧化酶法测定入组患者血浆甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-c)和低密度脂蛋白胆固醇(LDL-c)的水平。结果脑梗死患者nNOS基因rs9658581的等位基因频率与TC呈显著性相关(χ2=4.583,P=0.032)。携带G等位基因个体的总胆固醇水平(TC)明显高于携带A等位基因的个体。结论脑梗死患者TC的水平与NOS1基因rs9658581多态性可能有关。Objective To investigate the genetic association between the NOS1 gene and lipid metabolism in cerebral infarction.Methods 605 patients with cerebral infarction were recruited into this study. SNP rs9658281,a G to A base change located in intron 2 of the gene,and SNP rs2682820,an A to C base change located in intron 16 of the gene were used as genetic markers.PCR-based restriction fragment length polymorphism analysis was applied to genotype rs9658281 （MSP I site） and rs2682820 （Hae Ⅲ site）.The levels of plasma triglyceride,total cholesterol,LDL-cholesterol and HDL-cholesterol were detected by oxidation enzyme method.Results The levels of total cholesterol showed significant difference in G allele than in A allele in rs9658281 （χ2=4.583,P=0.032）.But in rs2682820,the levels of total cholesterol,triglyceride,HDL-cholesterol and LDL-cholesterol showed no significant difference in C allele than in A allele.Conclusions The present study suggests that the NOS1 gene is likely to contribute to the level of total cholesterol in cerebral infarction.

关 键 词：神经元型一氧化氮合酶基因(NOS1) 总胆固醇(TC) 单核苷酸多态性(SNP) 

分 类 号：R743.3[医药卫生—神经病学与精神病学]