细胞因子对活化淋巴细胞粘附血管内皮细胞影响机理的研究  

Effects of cytokine on adherence of activated lymphocyte to vascular endothelial cells

作　　者：侯桂华[1] 韩建奎[1] 张庆殷[1] 宋静[1] 卢红霞 

机构地区：[1]山东医科大学微生物教研室

出　　处：《中国免疫学杂志》1999年第3期114-116,共3页Chinese Journal of Immunology

摘　　要：目的：探讨细胞因子对ｒＩＬ２活化的脐血淋巴细胞粘附脐静脉血管内皮细胞（ＨＵＶＥＣ）的影响机理。方法：计数粘附率和ＡＰＡＡＰ法测细胞表型。结果：ｒＩＬ２活化淋巴细胞粘附ＨＵＶＥＣ的能力明显大于未刺激组，前者的粘附率是５６．５２％，后者是２４．５４％。ｒＩＬ１、２、８、ｒＴＮＦα可明显增强其粘附，而ｒＩＬ１０使粘附率明显下降至３８．１２％；ｒＩＬ１、８、ｒＴＮＦα增强ＣＤ４＋细胞的粘附，ｒＩＬ１０抑制ＣＤ４＋、ＣＤ５６＋的粘附。结论：活化淋巴细胞粘附ＨＵＶＥＣ的作用明显增强，ｒＩＬ１、２、８、１０和ｒＴＮＦα对这一过程具有重要的调节作用。Objective:To explore the effects of different cytokine on the adherence of rIL 2 activated umbilical lymphocytes to HUVEC(human umbilical vein endothelial cells).Methods :Activated umbilical lymphocytes were laid on the surface of the cultured HUVEC monolayer. After a certain period of time,the adherence rate was determined. The phenotypes of adherent lymphocytes were studied with APAAP method.Results:rIL 2 activated lymphocytes exhibited a stronger adherence to HUVEC than that of control group.rIL 1(10 U/ml) rIL 2(1 000 U/ml ) rIL 8(1 mg/ml)and rTNF α(250 U/ml) could promote the adherence of rIL 2 activated lymphocytes,but rIL 10 decreased the adhesion rate to 38.12%.Conclusions:The adherent activity of activated lymphocytes is obviously stronger than that of unactivated ones and cytokine may play an important role in regulating lymphocyte adherence to vascular endothelium.

关 键 词：血管内皮细胞 活化淋巴细胞 细胞因子 肿瘤 

分 类 号：R730.51[医药卫生—肿瘤] R392.1[医药卫生—临床医学]