骨巨细胞瘤增殖细胞核抗原与bc1-2基因表达及凋亡的检测研究  

Analysis on expression of PCNA and bc1-2 oncogene and apoptosis in giant cell tumor of bone

作　　者：李月白[1] 李家谋[2] 王义生 

机构地区：[1]河南医科大学基础医学院生化教研室,郑州450052 [2]河南医科大学附属第一医院骨科

出　　处：《中华实验外科杂志》1999年第2期138-139,共2页Chinese Journal of Experimental Surgery

基　　金：河南省科委科技攻关计划基金!No.951150121

摘　　要：探讨骨巨细胞瘤（ＧＣＴ）的细胞学性质、肿瘤发生以及生物学行为。方法用免疫组化（标记的链霉卵白素生物素──ＬＳＡＢ）法检测５１例ＧＣＴ中ＰＣＮＡ和ｂｃ１－２蛋白表达，并用ＴＵＮＥＬ法检测其凋亡细胞。结果全部标本ＰＣＮＡ阳性表达，随病理分级增加，ＰＣＮＡ表达有增高趋势。ｂｃ１－２在Ⅰ与Ⅲ级及Ⅰ与Ⅱ＋Ⅲ级间表达差异有显著性意义。ＧＣＴ中多为单核基质细胞凋亡，ＧＣＴ的凋亡率（ＡＩ）为０．８６％，各级间及ＰＣＮＡ标记指数间ＡＩ的差异无显著性意义，ＧＣＴ的ＡＩ与ｂｃ１－２表达呈显著负相关，ｂｃ１－２表达与否的ＡＩ差异有显著性。结论ＧＣＴ是以增殖为特征的肿瘤；ｂｃｌ－２在ＧＣＴ发生发展中可能起一定的作用。Objective To study the features of cytology, oncogenesis and biological behavior of giantcell tumor (GCT) of bone. Method The expression of PCNA and bc1-2 protein was detected by using immunohistochemical LSAB technique and cell apoptosis using TUNEL method in 51 cases of GCT. Accordingto Jaffe's classification, 26 cases were classified in grade Ⅰ, 16 cases in grade Ⅱ, and 9 cases in grade Ⅲ.Result The expression of PCNA was positive in all samples, but PCNA no staining in multinuclear giantcells. The proportion of positive PCNA labeled cells tended to be more with a higher pathological grade. Thepositive rate of bcl-2 expression was 33. 3% in GCT with the differences between Ⅰ and Ⅲ orⅠ and Ⅱ＋Ⅲgrade being statistically significant (P<0.05). Apoptosis mainly occurred in mononucleate stromal cells ofGCT, rarely in multinuclear giant cells. Apoptosis index (AI) of GCT was 0. 86%. No significant differencein the AI between pathological grades and between PCNA labeling index. There was a negative correlation between AI and positive expression of bc1-2 protein in GCT (P<0.05). There was a significant diffelence inthe. AI between bc1-2 positive and negative expression in GCT (P<0.05). Conclusion GCT is a tumorwith the proliferative characteristic. bcl-2 gene may play a role in the development of GCT.

关 键 词：骨巨细胞瘤 骨肿瘤 bcl-基因 PCNA 

分 类 号：R738.102[医药卫生—肿瘤]