雌激素对大鼠胰腺腺泡细胞癌癌前病变影响的实验研究  

The experimental study the effect of estrogen on precancerous lesion of rats' pancreatic acinar cellularcancer induced by Azaserine

作　　者：徐峰[1] 寿楠海[1] 姜希宏[1] 曹伟[2] 徐克森[1] 

机构地区：[1]山东医科大学附属医院普外科,济南250012 [2]山东医科大学基础医学院药理学教研室

出　　处：《中华实验外科杂志》1999年第2期159-160,共2页Chinese Journal of Experimental Surgery

摘　　要：了解雌激素对大鼠胰腺腺泡细胞癌癌前病变及其Ｋ－ｒａｓ基因突变的影响。方法雄性Ｗｉｓｔａｒ大鼠单次腹腔注射重氮乙酰丝氨酸（ａｚａｓｅｒｉｎｅ）３０ｍｇ／ｋｇ体重，部分大鼠２个月后开始每２周内肌肉注射成酸雌二醇０．１８ｍｇ／１００ｇ体重，４个月后实验结束常规取胰腺组织行光镜和电镜检查，并应用ＰＣＲ－ＳＳＣＰ法检测Ｋ－ｒａｓ基因突变情况。结果单纯给予Ａｚａｓｅｒｉｎｅ的大鼠胰腺腺泡细胞癌癌前病变（ＡＡＣＮ）发生率１００％，Ｋ－ｒａｓ基因突变率４２．１％；经雌激素处理后，ＡＡＣＮ发生率９２．４％，Ｋ－ｒａｓ基因突变率２５．６％。结论Ａｚａｓｅｒｉｎｅ可成功诱导大鼠胰腺腺泡细胞癌癌前病变，Ｋ－ｒａｓ基因突变在此过程中属早期事件。雌激素在一定程度上可抑制该癌癌前病变的发生发展。Objective To set up the animal model of precancerous lesion of rats' pancreatic acinarcellular cancer, learn the effect of estrogen on this lesion's biological feature and K-ras gene mutation.Method Male Wistar rats were injected Azaserine 30 mg/kg (ip). Some rats received estradiol-17 βValerate (0. 18 mg/100 g 2 weeks, im). Four months later, the rats'pancreas was autopsied and K-rasgene mutation was detected by PCR-SSCP method. Result The incidence of AACN was 100%. Theincidence of K-ras gene mutation was 42.1% in rats without estrogen and 25.6% with estrogen. Conclusion Azaserine could induce precancerous lesion of rats' pancreatic acinar cancer successfully. K-rasgene mutation was an early event during this process. Estrogen could inhibit the development of precancerous lesion to some degree.

关 键 词：重氮乙酰丝氨酸 胰腺癌 雌激素 K-RAS 癌前病变 

分 类 号：R735.902[医药卫生—肿瘤]