胆囊结石患者胆固醇酯转移蛋白基因多态性研究  

Polymorphism at Cholesteryl Ester Transfer Protein Gene Loci in Patients with Gallstone

作　　者：张明仪[1,2] 肖路加 林琦远[1,2] 魏家宾[1,2] 舒晔 刘嘉林 

机构地区：[1]华西医科大学附属第一医院普外科 [2]成都军区总医院普外科

出　　处：《华西医科大学学报》1999年第1期68-71,共4页Journal of West China University of Medical Sciences

摘　　要：为揭示胆囊结石病与遗传因素的关系，运用聚合酶链反应技术（ＰＣＲ）和基因限制性片段长度多态性分析法（ＲＦＬＰｓ）对１０４例胆囊结石患者和６８例健康人进行了研究。结果显示：胆囊结石组Ｂ１等位基因频率５２．３％，Ｂ１Ｂ１基因型频率２８．８％，大于对照组Ｂ１等位基因频率３４．６％（Ｐ＜０．０５），Ｂ１Ｂ１基因型频率１１．７６％（Ｐ＜０．０５），胆囊结石组Ｂ２等位基因频率４７．７％，Ｂ２Ｂ２基因型频率２４．２％小于对照组Ｂ２等位基因频率６５．４％（Ｐ＜０．０５），Ｂ２Ｂ２基因型频率４２．６％（Ｐ＜０．０５）。胆囊结石组Ｈ１等位基因频率２０％，Ｈ１Ｈ１基因型频率５％，Ｈ２Ｈ２基因型频率６５％，Ｈ２等位基因频率８０％与对照组Ｈ１等位基因频率１７．９％，Ｈ１Ｈ１基因型频率２％，Ｈ２Ｈ２基因型频率６５．９％，Ｈ２等位基因频率８２．１％比较无显著性差异（Ｐ＞０．０５）。提示：Ｂ１等位基因是胆囊结石患者主导基因。Ｂ２等位基因是对照组的主导基因。Ｈ１及Ｈ２等位基因频率在胆囊结石患者及对照组中分布相同，无显著性差异（Ｐ＞０．０５）。This study was designed to reveal the association between the polymorphism of cholesteryl ester transfer protein (CETP) gene loci and gallbladder stone disease. By means of polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLEs) of CETP taqIB, BamHI genes were examined in 104 patients subjected to cholecystectomy for symptomatic gallstone disease and 68 healthy controls. The distribution of CETP taqIB polymorphism in the patients with gallstones differed significantly from that of the controls. The B 1 allele and B 1B 1 jects were more frequently revealed in patients with gallstones (0.523, vs 0.346, 0.288 vs 0.1176, P<0.05) . The B 2 allele and the B 2B 2 jects appeared in the controls more frequently (0.654 vs 0.477, 0.4264 vs 0.242, P<0.05 ). The H 1 allele, H 2 allele and H 1H 1 genotypic, H 2H 2 genotypic frequencies of the patients with gallstones were not significantly different from those of the controls ( P>0.05) . The results suggest that B 1 allele is the major gene in the patients with gallstones, and B 2 allele is more frequently revealed in the controls.

关 键 词：胆囊结石病 胆固醇酯转移蛋白 基因多态性 

分 类 号：R575.620.2[医药卫生—消化系统]