半乳糖基抗CD_3单抗-TIL体外趋肝性研究  

In Vitro Hepatic Targeting Tendency of Galactosyl anti CD 3 McAb TILS

作　　者：蒋平[1] 何生[1] 章崇杰[1] 庞其捷[1] 赵宗蓉[1] 宗志平 

机构地区：[1]华西医科大学附属第一医院普外科

出　　处：《华西医科大学学报》1999年第1期72-74,共3页Journal of West China University of Medical Sciences

基　　金：四川省科委应用基础研究基金

摘　　要：为增强肝癌肿瘤浸润淋巴细胞（ＴＩＬ）的趋肝性，制备了半乳糖基抗ＣＤ３单克隆抗体－ＴＩＬ（Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ－ＴＩＬ），将其与肝细胞共同孵育，在倒置显微镜下观察两者通过无唾液酸糖蛋白受体介导的作用情况。测定了半乳糖基抗ＣＤ３单抗（Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ）的糖密度以及Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ与ＴＩＬ的结合率。结果发现，Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ－ＴＩＬ与肝细胞有明显粘附现象。Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ的糖密度为６２．１８，Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ与ＴＩＬ的结合率为９７．９％。结果表明，Ｇａｌ－ａｎｔｉＣＤ３ＭｃＡｂ－ＴＩＬ在体外有良好的趋肝性，６２．１８的糖密度和９７．９％的结合率保证了ＴＩＬ的有效利用。This study was undertaken to enhance the hepatic targeting tendency of tumor infiltrating lymphocytes (TILs) and hence to lower the recurrence rate of primary liver cancer after hepatectomy. Galactosyl anti CD 3McAb TILs were prepared and then were incubated together with hepatocytes. Their interaction through asialoglycoprotein receptor mediated mechanism was observed under the inverted phase contrast microscope. The carbohydrate density of galactosyl anti CD 3McAb and the combining rate of galactosyl anti CD 3McAb with TILs were measured. The results revealed that galactosyl anti CD 3 McAb TIL obviously were adhered to hepatocytes. The carbohydrate density of galactosyl anti CD 3McAb was 62.18, and the combining rate of galactosyl anti CD 3McAb with TILs was 97.9%. The results suggested that in vitro hepatic targeting tendency of galactosyl anti CD 3McAb TILs was satisfactory, and that the carbohydrate density of 62.18 and the combining rate of 97.9% ensured the effective use of TILs.

关 键 词：肝肿瘤 TIL 抗CD3 单克隆抗体 趋肝性 

分 类 号：R735.702[医药卫生—肿瘤]