应用响应面法优化去氢表雄酮1α-羟基化的转化培养基  

Medium Optimization for 1α-Hydroxylation of Dehydroepiandrosterone by Response Surface Method

作　　者：刘佩卉[1] 刘新利[1] 鞠培殿[1] 张海涛[1] 

机构地区：[1]山东轻工业学院微生物工程山东省重点实验室,山东济南250353

出　　处：《食品与药品》2010年第7期256-261,共6页Food and Drug

基　　金：山东省自然科学基金资助(Y2008D18);山东省博士后创新项目专项基金资助(No.200903077)

摘　　要：目的优化斜卧青霉Penicillium decumbens ph-13转化去氢表雄酮(DHEA)为1α-羟基去氢表雄酮(1α-OH-DHEA)的转化培养基。方法在单因素实验的基础上,采用响应面分析法优化斜卧青霉ph-13转化DHEA的转化培养基,找出主要影响因素,经回归分析建立1α-OH-DHEA的转化率对培养基主要成分的二次回归模型。结果其回归方程的决定系数达到98.39%,得到的最佳培养基主要组成为:蔗糖4.75%、硝酸钠3.69%、硫酸镁0.31%、硫酸亚铁0.002%、硫酸锰0.001%。结论优化后的培养基中目标产物1α-OH-DHEA转化率达到18.83%。Objective To optimize the medium for transforming dehydroepiandrosterone（DHEA） to 1α-OH-DHEA by Penicillium decumbens ph-13.Methods On the basis of single factor test,the response surface method（RSM） was used to optimize the medium of transforming DHEA to 1α-OH-DHEA by Penicillium decumbens ph-13.The main in？ uential factors were obtained and the regression equation expressing the relationship between transformation rate and main components of medium was established by the regression analysis method.Results The R-squared in the model of regression equation was 98.39 %.The optimum medium components were sucrose 4.75 %,sodium nitrate 3.69 %,magnesium sulfate 0.31 %,ferrous sulfate 0.002 % and manganese sulfate 0.001 %.Conclusion In the optimized medium,the transformation rate of 1α-OH-DHEA can be up to 18.83 %.

关 键 词：斜卧青霉 去氢表雄酮 1α-羟基去氢表雄酮 培养基优化 响应面法 

分 类 号：Q93-3[生物学—微生物学]