大鼠肠缺血-再灌注损伤后细菌易位发生部位的实验研究  

The site of bacterial translocation after rat intestinal ischemia-reperfusion

作　　者：黄晓旭[1] 全竹富[1] 朱文强[1] 李秋荣[1] 黎介寿[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2010年第4期239-242,共4页Parenteral & Enteral Nutrition

基　　金：国家自然科学基金重点项目(30830098)

摘　　要：目的:探讨肠道缺血-再灌注损伤对不同肠段细菌易位的影响。方法:将大鼠随机分为对照组(行假手术)和肠道缺血-再灌注组(分别于再灌注后1、6和24 h取材)。检测空肠、回肠和结肠的细菌、内毒素易位情况,各段肠系膜静脉中的肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平。结果:对照组和肠道缺血-再灌注1 h组各肠段系膜淋巴结细菌培养均阴性,再灌注后24 h组空肠、回肠系膜淋巴结细菌易位率明显高于对照组和结肠系膜淋巴结(P<0.05);两组大鼠各段肠系膜静脉血培养、内毒素测定均为阴性;肠道缺血-再灌注后1 h和6 h组大鼠TNF-α浓度明显高于对照组(P<0.05);各段肠系膜静脉血IL-6浓度均明显高于对照组(P<0.05),再灌注后6 h组,空肠、回肠的肠系膜静脉血IL-6浓度明显高于结肠(P<0.05)。结论:肠道缺血-再灌注损伤可造成肠道细菌易位,空、回肠更易遭受肠缺血-再灌注损伤的打击,从而发生细菌易位。Objective： The study was designed to find out the effect of intestinal ischemia and reperfusion injury on gut barrier function and the site of bacterial translocation.Methods： Healthy male SPF SD rats were divided into two groups at random： group A[simple laparotomy（sham）,known as the control group] and group B[intestinal ischemia and reperfusion（I/R）].Bacterial translocation and the levels of endotoxin and cytokine in mesenteric circulation were measured separately in jejunum,ileum and colon and at 1,6 and 24 hours after I/R.Results： There was no bacteria in all groups in the mesenteric lymph nodes（MLN） at 1h after reperfusion.Colony counts of jejunum and ileum MLN in 24 h after reperfusion I/R in group B were significantly higher than that of colon MLN（P〈0.05）.No bacteria and endotoxin were detected in the blood from the mesenteric vein in all groups.Concentrations of cytokine TNF-α and IL-6 at 1,6 h after reperfusion in the I/R in group B was significantly higher than that in sham group（P〈0.05）.The concentration of IL-6 in mesenteric vein blood of jejunum and ileum was significantly higher than that of colon at 6h after reperfusion in the I/R group（P〈0.05）. Conclusion： Bacterial translocation can be caused by Intestinal I/R.Compared with the large intestine,the small intestine is easier to injury of intestinal I/R.

关 键 词：肠缺血-再灌注损伤 细菌易位 炎症因子 大鼠 

分 类 号：R363[医药卫生—病理学]