Genistin防治增生性玻璃体视网膜病变的研究  

Effect of genistin on proliferative vitreoretinopathy

作　　者：游江[1] 姜德咏[1] 

机构地区：[1]中南大学湘雅二医院眼科,长沙410078

出　　处：《中南大学学报（医学版）》2010年第7期749-753,共5页Journal of Central South University ：Medical Science

基　　金：湖南省自然科学基金(06JJ50073)~~

摘　　要：目的:研究Genistin对外伤性增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)的防治作用。方法:用自体富含血小板血浆玻璃体腔内注射制备兔外伤性PVR模型,并同时将二甲基亚砜、2μg或40μg genistin、1mg氟尿嘧啶各0.1 mL注入兔眼玻璃体内,在不同时间点用眼底镜检查眼底情况,进行PVR分级。第28天行40μg genistin组模型眼及正常眼ERG检查;同天将4组模型眼球取出制作病理标本,行光镜检查。结果:A组第10天出现视网膜脱离,PVR随着时间延长发展至更严重等级。40μg genistin和氟尿嘧啶组也发生PVR,但其严重程度低于对照组(P<0.05)。组织学检查40μggenistin组未发现明显视网膜形态改变,视网膜电图检查也未观察到显著功能变化。结论:Genistin玻璃体腔内注射是安全的,并能有效抑制兔外伤性PVR的发生发展。Objective To examine the effect of genistin on traumatic proliferative vitreoretinopathy（PVR） in rabbits.Methods Traumatic PVR was induced in pigmented rabbits by intravitreal injection of platelet rich plasma.The eyes then received an intravitreal injection of dimethyl sulfoxide（0.1 mL）,2 or 40 μg genistin（0.1mL）,and 1 mg fluorouracil（0.1 mL）,respectively to form 4 groups.The eyes were examined ophthalmoscopically on distinct days after the surgery and the stage of PVR was evaluated.The model eyes and normal eyes in the 40 μg genistin group carried ERG test on the 28th day.All model eyes in the 4 groups were observed by light microscope on the 28th day.Results In the control eyes,the retina was detached after 10 d,the PVR had progressed to higher stages with time.In the eyes injected 40 μg genistin or fluorouracil,the PVR also developed;however,the severity of PVR was lower than that in the control eyes.PVR was significantly inhibited in the 40 μg genistin group compared with the control eyes after 14 d（P0.05）.Histological examination of the genistin-treated eyes disclosed no morphological changes,and ERG analysis revealed no significant functional changes.Conclusion Intravitreal injection of genistin is safe and effective in reducing traumatic PVR in clinical treatment.

关 键 词：增生性玻璃体视网膜病变 视网膜色素上皮细胞 GENISTIN 兔 

分 类 号：R774.1[医药卫生—眼科]