灌胃与腹腔注射对小鼠自主活动的影响  

Effects of irrigation and intraperitoneal injection on locomotor activity of mice

作　　者：刘天雅[1] 洪宗元[1] 

机构地区：[1]皖南医学院定量药理研究所,药理学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2010年第4期241-243,共3页Journal of Wannan Medical College

基　　金：教育部科学技术研究重点项目(208058);安徽省自然科学基金项目(070413139)

摘　　要：目的:探讨灌胃(irrigation)与腹腔注射(intraperito-nealinjection)两种刺激对小鼠自主活动(locomotor activity)的影响,为评价药物中枢作用提供给药途径上的选择。方法:28只昆明种小鼠,随机分为灌胃组与腹腔注射组,每组14只。实验前,小鼠均置于自主活动记录仪中适应1h。第1d9∶00时,在无干扰下记录各小鼠的自主活动数,每次记录10min,连续3次。第2d9∶00时,灌胃组与腹腔注射组小鼠分别给予生理盐水(0.1ml/10g)灌胃与腹腔注射,操作完成后立即记录每只小鼠的自主活动数,记录方法同上。结果:灌胃与腹腔注射两种刺激均可使小鼠自主活动显著增加,持续约10min(P<0.05),但两组间差异不显著,无统计学意义(P>0.05)。结论:灌胃与腹腔注射两种刺激均可对小鼠自主活动产生影响,但影响程度相当。Objective：To investigate the stimulative effects on the locomotor activity of mice induced by irrigation and intraperitoneal injection for the evidence in evaluating the effect of a drug on the central nervous system.Methods：Twenty-eight Kunming mice were randomized into group of irrigation（ig,n=14） or intraperitoneal injection （ip,n=14）. All animals were habituated in the locomotion recording cage for 1h before the experiment. At 9：00 am of the first day,the locomotor activity of each mouse was recorded for 10 min,with 3 consecutive times. At 9：00 am of the second day,the animals in the two groups were given irrigation and intraperitoneal injection with 0.9 % NaCl saline at dose of 0.1 ml/10g,respectively,and the locomotor activity of each animal was kept immediately after the administration by the similar methods described above. Results：Two stimulations could significantly increase the locomotor activity of mice by 10 min（P0.05）,but no difference was found between the two groups（P0.05）. Conclusion：Both irrigation and intraperitoneal injection can relevantly stimulate the locomotor activity of mice,yet leading to the similar effect on the experimental animals.

关 键 词：小鼠 自主活动 灌胃 腹腔注射 

分 类 号：R-332[医药卫生]