生鲜乳中添加皮革水解蛋白的测定方法研究  

Study on Determination Method of Protein Hydrolysis in Raw Milk Added Leather

作　　者：刘学琴 徐栓朝 高建龙 刘维华 

机构地区：[1]宁夏水产研究所,宁夏银川750001 [2]银川兴庆区农牧局,宁夏银川750001 [3]宁夏动物食品质量安全检测中心,宁夏银川750001

出　　处：《乳业科学与技术》2010年第4期188-192,共5页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

摘　　要：L-羟脯氨酸是皮革水解蛋白构成的特征氨基酸,因此鉴定L-羟脯氨酸就是鉴定皮革水解蛋白。建立UV法测定生鲜乳中皮革水解蛋白,L-羟脯氨酸浓度在0μg/mL～2.0μg/mL范围呈良好的线性关系,R=0.9998。L-羟脯氨酸平均回收率为100.2%,猪皮粉平均回收率为98.1%,皮革粉平均回收率为99.9%。该方法回收率高,检出限低,工作曲线稳定,重复性好。L-hydroxyproline is the characteristic of leather hydrolyzed protein amino acid, the identification is the identification of L-hydroxyproline leather hydrolyzed protein.UV method established in the leather raw and fresh milk hydrolyzed protein, L-hydroxyproline concentration in the range of 0 μg/mL～2.0 μg/mL good linear relationship, R=0.9998.L-hydroxyproline average recovery was 100.2 %, pig powder average recovery was 98.1 %, and leather powder average recovery was 99.9 %.This method was high in recovery, low in detection limit, stable-working curve and good-repeating.

关 键 词：生鲜乳 皮革水解蛋白 方法研究 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]