真菌细胞溶壁酶的分离纯化及其酶学性质研究  

Purification and Properties of Lywallzyme from Trichoderma Longibrachiatum Rifai

作　　者：尹海滨 王婷 郑萍 

机构地区：[1]广州市元通医药科技有限公司,广东广州510610

出　　处：《广州化工》2010年第7期87-88,96,共3页GuangZhou Chemical Industry

基　　金：广东省广州市天河区科技局攻关项目(项目号082G091)

摘　　要：采用长梗木霉(Trichoderma Longibrachiatum Rifai)958-11菌株,经过发酵,分离提纯出一种真菌细胞溶壁酶,并对其进行酶学性质研究。长梗木霉培养,利用:(NH4)2SO4沉淀,sartobind S离子交换层析和Sephacryl S-200柱层析对该发酵液进行分离纯化,用担子菌做为酶反应的底物研究该酶的酶学性质。经发酵获得的酶液,可在最佳反应条件下,1～2h内产出原生质体105～106个/毫升酶液。酶反应最适温度为30℃,最适pH为5.4,可以在0.6mol/L的氯化钾或0.6mol/L的硫酸镁为等渗液的条件下,溶解部分担子菌的细胞壁,得到高活力的原生质体。Trichoderma Longibrachiatum Rifai 958-11 was applied to produce Lywallzyme by submerged fermentation.From the Sartobind S Ion Exchange Chromatography and Sephacryl S-200 Column Chromatography,lywallzyme was found,separated and purified.The relative molecular weights of the enzymes was determined and their most suitable reaction temperatures and pH values were ascertained.The lywallzyme can degrade fungal cell walls to produce protoplasts at pH 5.4 and 30℃.Under the optimum conditions,the protoplasts can be 105～106/mL enzyme.If in 0.6mol/L of potassium chloride or 0.6mol/L of magnesium sulfate as the isotonic solution,dissolved some cell walls of basidiomycets and got the protoplasts with high energy.

关 键 词：溶壁酶 长梗木霉 原生质体 

分 类 号：TQ927[轻工技术与工程—发酵工程]