自人脑胶质瘤cDNA文库克隆与鼠SH2┐B同源基因片段  

Cloning a Novel Gene Fragment Homologous to SH2 B from cDNA Library of Human Brain Glioblastoma

作　　者：吕立夏 徐磊 许蕾 李学礼 杨翠香 王尧 

出　　处：《上海铁道大学学报》1999年第3期9-11,共3页

摘　　要：目的自人脑胶质瘤文库克隆新的表达序列标签。方法以人脑胶质瘤文库为模板，利用生长休止特异基因的５′特异引物与载体特异引物组合进行ＰＣＲ扩增，对目的片段进一步克隆、测序。结果扩增出一个３２８ｂｐ的片段，同源性分析发现该片段与鼠ＳＨ２－Ｂ有８６％的同源性。结论ＳＨ２－Ｂ是生长激素信号转导途径中酪氨酸激酶ＪＡＫ２的底物，推测得到的基因片段可能介导胶质瘤的发生、发展过程中的某些信号转导途径，需克隆其ｃＤ－ＮＡ序列并研究其基因表达产物的功能方可证实。Objective It is to find a novel expressed sequence tags (EST) from a cDNA library of human brain glioblastoma.Methods PCR was carried out by using a 5′primer of growth arrest specific gene (Gas) with a pair of λ gt11 specific primers.The gene fragment of interest was cloned and sequenced.Results A 328 bp of EST was obtained and it shared 86% homology with SH2 B.Conclusion SH2 B was identified as a substrate of tyrosine kinase JAK2 in growth hormone signaling.Whether this new EST which has high homology with SH2 B mediates an unidentified signal transduction pathway in the development of human brain glioblastoma should be determined by cloning its intact cDNA sequence and investigaing its function of gene product.

关 键 词：脑肿瘤 CDNA文库 克隆 脑质瘤 SH2-B Gas 

分 类 号：R739.41[医药卫生—肿瘤]