PCR和原位杂交检测食管癌组织中人乳头状瘤病毒  

Detection of human papillomavirus(HPV) on esophageal carcinoma by PCR and in－situ hybridization

作　　者：吕丽春[1] 沈忠英[1] 游绍进[1] 沈健[1] 蔡唯佳[1] 

机构地区：[1]汕头大学医学院病理教研室,广东汕头515031

出　　处：《癌症》1999年第2期162-164,共3页Chinese Journal of Cancer

摘　　要：目的：探索汕头高发区食管癌的发病是否与HPV感染有关。方法：用L1区共用引物PCR和HPV18DNA探针原位杂交技术对55例新鲜食管瘤标本进行检测。结果：PCR和原位杂交的HPV阳性检出率分别为92．72％和81．80%，癌旁粘膜HPV检出率高于癌组织（P＜0．05）。而且原位杂交提示不同病变杂交信号分布特征不同：癌旁“正常”和单纯增生上皮呈层状分布，原位癌层状分布特征消失，阳性细胞弥散全层，浸润癌呈区域性阳性。结论：HPV感染可能是食管癌的病因之一。Purpose: To search wether HPV infection is associated with esophageal carcinoma. Method: L1 concensus primers in the Polymerase Chain Reaction(PCR) and HPV 18 probe in In－situ Hybridization(ISH) were used for the detection of HPV infection from each of 55 fresh esophageal carcinoma secimens. Results:The Positive rate of esophageal cancer tissue was 92. 72% and 81. 80% respectively by PCR and ISH. Paracaroinoma mucosa had higher positive rate than that of cancer (P < 0. 05). Moreover, HPV hybridization signals were related to the epithelial lesions. HPV 18 hybridization signals were distributed in layers in normal and simple hyperplasia epithelium. The spots were diffusively distributed in the whole epithelium and layer characteristics disappeared in carcinoma in－situ. Hybridization signals were focally positive in invasive cancer. ConcluSion: It suggested that HPV infection might be one of the causes of esophageal carcinoma.

关 键 词：食管肿瘤 HPV 聚合酶链反应 原位杂交 

分 类 号：R735.104[医药卫生—肿瘤] R373.9[医药卫生—临床医学]