多重耐药基因在睫状体上皮细胞容积调节中的作用  

The role of the multiple drug resistance gene in the volume regulation of ciliary epithelial cells

作　　者：陈丽新 王立伟 

机构地区：[1]英国卡的夫大学生物学院生理系

出　　处：《中国病理生理杂志》1999年第4期365-368,共4页Chinese Journal of Pathophysiology

摘　　要：目的：研究多重耐药（ＭＤＲ１）基因与细胞容积调节的关系。方法：用反义寡核苷酸阻抑牛眼睫状体非色素上皮细胞ＭＤＲ１基因表达，在激光共聚焦显微镜下检测ＭＤＲ１基因反义寡核苷酸（ＭＤＲ－ａｎｔｉ）的导入，用反射／散射技术测量由低渗液引起的细胞容积变化。结果：阳离子脂质体促进ＭＤＲ－ａｎｔｉ导入细胞，ＭＤＲ－ａｎｔｉ导入量与剂量呈依赖性增强关系（ｒ＝０９７，Ｐ＜００１）。人ＭＤＲ－ａｎｔｉ使低渗液引起的细胞调节性容积减小反应延迟、缓慢，容积恢复不完全（６０％），而鼠ＤＭＲ－ａｎｔｉ没有此作用。结论：人ＭＤＲ－ａｎｔｉ抑制细胞调节性容积减小，提示ＭＤＲ１基因参与细胞容积调节。AIM:The relationship between multiple drug resistance (MDR1) gene and the volume regulation was investigated.METHODS:Antisense “knock-down” approach was used to inhibit MDR1 gene expression in bovine nonpigmented ciliary epithelial cells. The uptake of MDR1 antisense oligonucleotides (MDR-anti) was detected by a confocal microscopy. Volume changes following exposure to hypotonic solution were measured using a light reflection/scattering technique. RESULTS:The transfection reagent lipofectin facilitated the uptake of MDR-anti. The uptake of hmuan MDR-anti was directly proportional to the external concentration of antisense. There was a linear relationship between them ( r =0 97, P <0 01). The regulatory volume decrease (RVD) induced by hypotonic solution was delayed and slowed down by incubating the cells with human MDR-anti. The volume of the cells only partially recovered (60%). There was no effect of mouse MDR-anti on the volume regulation. CONCLUSION:The specific MDR-anti inhibited the RVD and this suggests that MDR1 gene be involved in the volume regulation of cells.

关 键 词：眼 睫状体 上皮细胞容积 多药耐药基因 

分 类 号：R773.302[医药卫生—眼科]